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NanoCellBiology of Secretion: Imaging Its Cellular and Molecular Underpinnings: SpringerBriefs in Biological Imaging

Autor Bhanu P. Jena
en Limba Engleză Paperback – 19 ian 2012
Understanding live cells at the single molecule level is the most important and single major challenge facing biology and medicine today. Over the past 15 years, there has been a renewed understanding of living cells at the molecular level. Atomic Force Microscopy, Laser Force Microscopy, single secretory vesicle patch clamp studies, highresolution electron microscopy, and x-ray diffraction, are some of the tools now being used to unravel the intricacies of a living cell at the molecular level. Opening with an explanation of Materials and Methods, NanoCellBiology then moves through discussions of porosome discovery, calcium and SNARE-induced fusion, and vesicle swelling before winding up in a final chapter of conclusions and future studies. Succinctly packaged as SpringerBrief, this book is a must for those studying or conducting research in cell biology, biochemistry or nanobiology/nanotechnology. This book will be invaluable to faculty & graduate students involved in Nano Courses; Cell Biology Courses; Biophysics Courses; and Biochemistry Courses as well as practicing Cell Biologists, Biochemists and BioPhysicists.
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Specificații

ISBN-13: 9781461424376
ISBN-10: 1461424372
Pagini: 73
Ilustrații: XII, 70 p. 38 illus., 33 illus. in color.
Dimensiuni: 155 x 235 x 13 mm
Greutate: 0.14 kg
Ediția:2012
Editura: Springer
Colecția Springer
Seria SpringerBriefs in Biological Imaging

Locul publicării:New York, NY, United States

Public țintă

Professional/practitioner

Cuprins

Understanding live cells at the single molecule level is the most important and single major challenge facing biology and medicine today. Over the past 15 years, there has been a renewed understanding of living cells at the molecular level. Atomic Force Microscopy, Laser Force Microscopy, single secretory vesicle patch clamp studies, highresolution electron microscopy, and x-ray diffraction, are some of the tools now being used to unravel the intricacies of a living cell at the molecular level. Opening with an explanation of Materials and Methods, NanoCellBiology then moves through discussions of porosome discovery, calcium and SNARE-induced fusion, and vesicle swelling before winding up in a final chapter of conclusions and future studies. Succinctly packaged as SpringerBrief, this book is a must for those studying or conducting research in cell biology, biochemistry or nanobiology/nanotechnology. This book will be invaluable to faculty & graduate students involved in Nano Courses; Cell Biology Courses; Biophysics Courses; and Biochemistry Courses as well as practicing Cell Biologists, Biochemists and BioPhysicists.
Table of contetns:
Introduction.- Materials & Methods.- Porosome Discovery.-  Calcium & SNARE-Induced Membrane Fusion.- SNAREs need to reside in opposing membrane.- Membrane curvature dictates SNARE size.- Disassembly of membrane-associated SNARE complex.- CD spectroscopy confirm membrane requirement for t-/v-SNARE assembly.- SNAREs br
ing opposing bilayers closer for calcium bridging.- Membrane lipids influence SNARE complex assembly-disassembly.- Vesicle swelling and content expulsion during cell secretion.- Secretory vesicle swelling is required for intravesicular content expulsion.- Molecular mechanism of secretory vesicle swelling.- Presence of beta receptors at the secretory vesicle membrane.- Conclusion and future studies.​

Recenzii

“This is a reference discussing the molecular mechanisms of the following problems: Exocytocis, secretion of hormones in pancreatic and excitable cells. … This reference is of use to physiologists, biochemists, cell biologists, and membrane experts.” (Joseph J. Grenier, Amazon.com, September, 2015)