This report provides a comprehensive assessment of the state of natural resources at Fort Donelson National Battlefield (FODO). It also addresses sets of stressors that threaten these resources and the biological integrity of habitats in the park. This assessment focuses on vital signs outlined by the Cumberland Piedmont Inventory and Monitoring (I&M) Network (CUPN), and on attributes relevant to the park's natural resources. Assessed attributes are roughly organized into broad groups of resources as follows: air quality, water quality, vegetation, animal communities, and landscape dynamics.