Oligonucleotide Array Sequence Analysis de Martina K. Moretti

Oligonucleotide Array Sequence Analysis

Martina K. Moretti

Lorenzo J. Rizzo

Oligonucleotides are short sequences of nucleotides (RNA or DNA), typically with twenty or fewer bases. Automated synthesisers allow the synthesis of oligonucleotides up to 160 to 200 bases. The length of a synthesised base is usually denoted by 'mer' (from 'Greek' meros "part"). For example, a fragment of 25 bases would be called a 25-mer. Oligonucleotides are often used as probes for detecting complementary DNA or RNA because they bind readily to their complements. Examples of procedures that use oligonucleotides are DNA microarrays, Southern blots, fluorescent in situ hybridisation (FISH), and the synthesis of artificial genes. This book brings together leading research in the field from around the globe.

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Cuprins

Preface; Visible Haplotype-TagSNP Typing Array Device for Human Radiation Sensitivity-Associated Genes; Specificity of Oligonucleotide Microarray Hybridization: Effectors and Their Effects; Stem Cell-based Therapy Approaches for Parkinsons Disease: Progress and Perspectives; The Clinical Relevance of Gene Expression Profiles in Peripheral Blood Mononuclear Cells; Clustering Time-Series Gene Expression Data; Recent Advances in Microbial Discovery Using Metagenomics, DNA Microarray, Biosensors, Molecular Subtyping, and Phage Recombinant Probes; Statistical Analysis of Tiling-path Microarrays; Application of Microarray in Rat Model of Alcoholic and Non-Alcoholic Liver Disease; Biological Interpretation in Oligonucleotide Array Sequence Analysis Based on Molecular Networks ; On Line Tools for Presentation and Analysis of Plant Microarray Data; Methods, Strategies and Facts for Improving the Analysis of Genetic Variations Using DNA Arrays; Improving Detection of Differentially Expressed Genes in Microarrays Using More Efficient Statistics, the Local Optimal Fudge Factor and a Two-Stage FDR Correction; Basic Approaches to Gene/Exon Level Data Analysis: 3'IVT and Exon 1.0 ST Microarray Platforms ; Simultaneous Qualitative and Quantitative Identification of Multiple Targets by Polymerase Chain Reaction on Microarray ; Positional Weight Matrix as a Sequence Motif Detector; Quality Control Methods for Oligonucleotide Microarrays; Applications of Qiagen QIAplex Technology in Clinical Multiplex Assays; Oncology Applications of Microarray Technology Detecting Small Genomes Variability; Index.