Located along Florida's southwest Gulf coast in Lee and Charlotte counties, the J.N. "Ding" Darling National Wildlife Refuge (NWR) Complex includes Pine Island, Matlacha Pass, Island Bay, Caloosahatchee, and J.N. "Ding" Darling NWRs (Figure 1). The Pine Island, Matlacha Pass, Island Bay, and Caloosahatchee refuges were each established with the primary purpose as a preserve and breeding ground for native birds.