Practical Handbook on the 3Rs in the Context of the Directive 2010/63/EU provides updated information on the EU Directive 2010/63/EU, which is the European Union legislation that protects animals being used in research. EU Directive 2010/63/EU is the European Union (EU) legislation 'on the protection of animals used for scientific purposes' and is one of the most stringent ethical and welfare standards worldwide.

• Closes a gap in scientific literature by addressing the need for clear guidance in walking through the multifaced universe of 3Rs
• Offers a useful starting point for readers and scientist who approach the 3Rs for the first-time
• Gives insights into the harmonization of the animal research legislation across countries