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Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice

Autor Fatma Sarsu, Abdelbagi M.A. Ghanim, Priyanka Das, Rajeev N. Bahuguna, Paul Mbogo Kusolwa, Muhammed Ashraf, Sneh L. Singla-Pareek, Ashwani Pareek, Brian P. Forster, Ivan Ingelbrecht
en Limba Engleză Paperback – 14 feb 2019
This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted – the seedling and flowering stages – with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly.

The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance. 
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Specificații

ISBN-13: 9783030084325
ISBN-10: 3030084329
Pagini: 39
Ilustrații: XII, 39 p. 16 illus., 10 illus. in color.
Dimensiuni: 155 x 235 mm
Greutate: 0.09 kg
Ediția:Softcover reprint of the original 1st ed. 2018
Editura: Springer International Publishing
Colecția Springer
Locul publicării:Cham, Switzerland

Cuprins

General Introduction.- Screening Protocols for Heat Tolerance in Rice at the Seedling and Reproductive Stages.- Validation of Screening Protocols for Heat Tolerance in Rice.- Conclusion.- References.

Notă biografică

Fatma Sarsu
Plant Breeding and Genetics Section
Joint FAO/IAEA Division
Vienna, Austria
 
Abdelbagi M.A. Ghanim
Plant Breeding and Genetics Laboratory
Joint FAO/IAEA Division
Vienna, Austria
 
Priyanka Das
School of Life Sciences
Jawaharlal Nehru University
New Delhi, India
 
Rajeev N Bahuguna
School of Life Sciences Jawaharlal Nehru University
New Delhi, India
 
Paul Mbogo Kusolwa
Sokoine University of Agriculture
Morogoro, United Republic of Tanzania
 
Muhammed Ashraf
Nuclear Institute for Agriculture and Biology (NIAB)
Faisalabad, Pakistan
 
Sneh Lata Singla-Pareek
Plant Molecular Biology Group International Centre for Genetic Engineering and Biotechnology
New Delhi, India
 
Ashwani Pareek
School of Life Sciences
Jawaharlal Nehru University
New Delhi, India
 
Brian P. Forster
Plant Breeding and Genetics Laboratory 
Joint FAO/IAEA Division
International Atomic Energy Agency 
Vienna, Austria

Ivan Ingelbrecht
Plant Breeding and Genetics Laboratory
Joint FAO/IAEA Division
International Atomic Energy Agency 
Vienna, Austria


Textul de pe ultima copertă

This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted – the seedling and flowering stages – with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly.

The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance. 

Caracteristici

Open Access provided with a grant from the International Atomic Energy Agency Provides robust, user-friendly protocols for effective pre-field screening of mutant rice for enhanced heat stress tolerance Designed for use by plant breeders who need rapid screens to process large mutant populations, including segregating populations, advanced generations and germplasm collections The described protocols could be adapted to screen for heat stress tolerance in other crops