This book is a detailed guide that provides insights into the preparation and application of nano-materials using macromolecules of natural product origin. The book covers various natural macromolecules such as chitin, chitosan, cellulose, lignin, and proteins and their conversion into nano-materials for different applications. The book describes the latest developments and techniques for the preparation of nano-materials from natural macromolecules, such as enzymatic degradation, chemical modification, and electrospinning. Furthermore, it discusses the unique physicochemical properties of these nano-materials, such as biocompatibility, biodegradability, and excellent mechanical properties, that make them suitable for various biomedical, environmental, and industrial applications. In addition, the book explores the applications of nano-materials from natural macromolecules in drug delivery, tissue engineering, biosensors, wastewater treatment, and packaging. It provides a comprehensive overview of the latest research studies and commercialization prospects of these nano-materials. Overall, "Preparation of Nano-materials from Natural Macromolecules" is a valuable resource for students, researchers, and professionals in the fields of materials science, nano-technology, biotechnology, and environmental science. It provides a detailed understanding of the preparation, properties, and applications of nano-materials from natural macromolecules, which will help in developing sustainable and eco-friendly technologies for various applications.