The second edition of this book has been extended to include new information and developments in the preservation of fungi, but maintains the coverage of methodology presented in the first edition published in 1983. The book provides a guide to the selection of suitable preservation procedures, information on the growth and maintenance of fungi, and the background to manage a collection of microorganisms both safely and effectively. The methods described are those in operation or experimented upon at the International Mycological Institute, and cover both simple and complex techniques. These methods are of use in all situations where living cultures of fungi are required, whether in teaching and research or in industry.