Primary and Secondary Metabolism of Plants and Cell Cultures III de J. Schripsema

Primary and Secondary Metabolism of Plants and Cell Cultures III: Proceedings of the workshop held in Leiden, The Netherlands, 4–7 April 1993

J. Schripsema

R. Verpoorte

en Limba Engleză Paperback – 14 oct 2012

Since 1984 and 1988, when meetings were held on the topic of primary and secondary metabolism of plant cell cultures, there has been a clear shift of the focus of ongoing research. While the cell culture itself and the production of secondary metabolites and the biosynthetic pathways and the activity of enzymes were major topics, now these aspects are linked with genes, i.e. molecular biology becomes more prominent. This state-of-the-art book has contributions on such subjects as fermentation, enzymology of secondary metabolism, catabolism of secondary metabolites, elicitation of pathways and genetic modification of metabolic pathways. It includes contributions on the most recent achievements in the research on among other things tropane and indole alkaloids, phenolics, (iso)flavonoids, terpenes and cardenolides. It is an excellent review of the progress made in the past years and a perspective on the future developments.

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Ajmalicine production by cell cultures of Catharanthus roseus: from shake flask to bioreactor.- Production of steroidal alkaloids by hairy roots of Solanum aviculare and the effect of gibberellic acid.- Cyclodextrins as a useful tool for bioconversions in plant cell biotechnology.- Embryogenesis of photoautotrophic cell cultures of Daucus carota L..- Semicontinuous cultivation of photoautotrophic cell suspension cultures in a 20 I airlift- reactor.- Studies on the relationship between ploidy level, morphology, the concentration of some phytohormones and the nicotine concentration of haploid and doubled haploid tobacco (Nicotiana tabacum L.) and NICA plants.- Influence of exogenous hormones on the growth and secondary metabolite formation in transformed root cultures.- Molecular cloning and expression of key enzymes for biosynthesis of cysteine and related secondary non-protein amino acids.- Thiophene biosynthesis in Tagetes roots: molecular versus metabolic regulation.- Regulatory mechanisms of biosynthesis of betacyanin and anthocyanin in relation to cell division activity in suspension cultures.- The biosynthesis of rosmarinic acid in suspension cultures of Coleus blumei.- The biosynthetic pathway of the S-alk(en)yl-L-cysteine sulphoxides (flavour precursors) in species of Allium.- Elicitor induced secondary metabolism in Ruta graveolens L. Role of chorismate utilizing enzymes.- Constitutive and elicitation induced metabolism of isoflavones and pterocarpans in chickpea (Cicer arietinum) cell suspension cultures.- Regulation of isoflavonoid metabolism in alfalfa.- Regulation of phenylalanine ammonia-lyase genes in carrot suspension cultured cells.- Accumulation of anthraquinones in Morinda citrifolia cell suspensions. A model system for the study of the interaction between secondary and primary metabolism.- Calystegines as a new group of tropane alkaloids in Solanaceae.- Esterification reactions in the biosynthesis of tropane alkaloids in transformed root cultures.- Characterization of Coptis japonica cells with different alkaloid productivities.- Traits of transgenic Atropa belladonna doubly transformed with different Agrobacterium rhizogenes strains.- Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus.- Catharanthine and ajmalicine synthesis in Catharanthus roseus hairy root cultures. Medium optimization and elicitation.- A novel 2-oxoglutarate-dependent dioxygenase involved in vindoline biosynthesis: characterization, purification and kinetic properties.- Are tissue cultures of Peganum harmala a useful model system for studying how to manipulate the formation of secondary metabolites?.- Breakdown of indole alkaloids in suspension cultures of Tabernaemontana divaricata and Catharanthus roseus.- The cell culture medium - a functional extracellular compartment of suspension- cultured cells.- Secondary metabolites in hairy root cultures of Leontopodium alpinum Cass. (Edelweiss).- Glycosylation in cardenolide biosynthesis.- Enzymes in cardenolide-accumulating shoot cultures of Digitalis purpurea L..- Enzymes involved in the metabolism of 3-hydroxy-3-methylglutaryl-coenzyme A in Catharanthus roseus.- Regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase by wounding and methyl jasmonate: implications for the production of anti-cancer alkaloids.