Cellulase is a multienzyme system responsible for hydrolysis of cellulose to glucose. It is commercially very important enzyme for the generation of glucose from lignocellulosic waste. Production of glucose is commercially viable process for the direct conversion of cellulosic material to glucose. Cellulase can be synthesized and purified by the growth of A. niger and other microbes under optimized condition which could be used for the production of glucose, ethanol etc from lignocellulosic waste for the benefit of mankind.