Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols
Editat de Paula Meleadyen Limba Engleză Paperback – 12 feb 2024
- Explores the most common questions new researchers have
- Guides readers to properly design the workflow for successful integration of mass spectrometry into protein biochemical analyses
- Provides examples of sample preparation for a number of different materials, mammalian cells, and others
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Specificații
ISBN-13: 9780323903950
ISBN-10: 0323903959
Pagini: 240
Ilustrații: 80 illustrations (50 in full color)
Dimensiuni: 191 x 235 x 19 mm
Greutate: 0.51 kg
Editura: ELSEVIER SCIENCE
ISBN-10: 0323903959
Pagini: 240
Ilustrații: 80 illustrations (50 in full color)
Dimensiuni: 191 x 235 x 19 mm
Greutate: 0.51 kg
Editura: ELSEVIER SCIENCE
Public țintă
Cell biologists, Biochemists, Molecular Biologists, Biomedical Engineers at Ph.D level and above, mostly in academia, Biophysicists and Structural Biologists. Clinicians and Research physicians, Industry-based proteomics researchersCuprins
Section I. Introduction
1. Book introduction
2. MS Proteomic Overview
Section II. Sample Preparation
3. Sample preparation from mammalian cell lines
4. Sample preparation for proteomics and MS from clinical tissue
5. Sample preparation for proteomics and MS from biological fluids, e.g. serum/plasma from patient blood
6. Sample preparation for proteomics and MS from bacterial and fungal samples
7. Sample preparation for proteomics and MS from urine samples including exosome isolation
8. Sample preparation for proteomics and MS from animal samples
9. Subcellular fractionation methods for proteomic analysis
Section III. Protein Digestion
10. In gel digestion for mass spectrometry
11. In solution digestion for mass spectrometry
Section IV. MS Proteomic Analysis Protocols
12. Protocols for Label-free LC-MS/MS proteomic analysis
13. Protocols for TMT labelled LC-MS/MS analysis
14. Phosphorylation enrichment for phosphoproteomic analysis
15. Ubiquitination enrichment for ubiquitinome PTM analysis
16. Glycosylation enrichment and analysis
17. LC-MS for protein identification and PTM analysis (e.g. methylation)
1. Book introduction
2. MS Proteomic Overview
Section II. Sample Preparation
3. Sample preparation from mammalian cell lines
4. Sample preparation for proteomics and MS from clinical tissue
5. Sample preparation for proteomics and MS from biological fluids, e.g. serum/plasma from patient blood
6. Sample preparation for proteomics and MS from bacterial and fungal samples
7. Sample preparation for proteomics and MS from urine samples including exosome isolation
8. Sample preparation for proteomics and MS from animal samples
9. Subcellular fractionation methods for proteomic analysis
Section III. Protein Digestion
10. In gel digestion for mass spectrometry
11. In solution digestion for mass spectrometry
Section IV. MS Proteomic Analysis Protocols
12. Protocols for Label-free LC-MS/MS proteomic analysis
13. Protocols for TMT labelled LC-MS/MS analysis
14. Phosphorylation enrichment for phosphoproteomic analysis
15. Ubiquitination enrichment for ubiquitinome PTM analysis
16. Glycosylation enrichment and analysis
17. LC-MS for protein identification and PTM analysis (e.g. methylation)