Widespread Glyphosate use as well as its persistent residue has been growing into a serious public health problem. In this research an attempt was made to decrease the Glyphosate and its metabolite level to the guideline values recommended by DOE. Coupled sequencing batch reactor (SBR) & adsorption mechanism has provided insight into the practical removal method for treatment of Glyphosate in wastewater. Artificial neural network (ANN) was used as a potential tools to monitor the treatment process.