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Techniques and Protocols in Molecular Biology

Autor Buddhi Prakash Jain
en Limba Engleză Paperback – mai 2025
Techniques and Protocols in Molecular Biology delivers detailed practical guidance for a variety of molecular techniques and procedures. Each chapter focuses on a different technique, including isolation techniques for DNA and RNA, gene cloning, PCR, DNA sequencing, ELISA and cell culture. These protocols all includes a detailed description of the technique along with the main principles, required equipment, and comprehensive steps to assist researchers with replicating the protocol and performing the techniques themselves.
This foundational reference is essential for students and researchers looking to enhance their understanding of key molecular techniques and reproduce them successfully in their own laboratory work.


  • Provides step-by-step instructions to guide researchers in carrying out a range of molecular biology techniques
  • Includes details of instrumentation, reagents and buffers necessary for each experiment
  • Features diagrams to help illustrate the procedures and techniques covered
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Specificații

ISBN-13: 9780443141607
ISBN-10: 0443141606
Pagini: 250
Dimensiuni: 191 x 235 mm
Editura: ELSEVIER SCIENCE

Cuprins

1. Isolation of Genomic DNA: From animal cells, tissues, plant cell, and bacteria 2. Isolation of Plasmid 3. Isolation of RNA 4. PCR (Polymerase Chain reaction) 5. Real-time (Quantitative) PCR 6. Electrophoresis 7. Capillary electrophoresis 8. Purification of Nucleic acid 9. Southern blotting 10. Northern blotting 11. ELISA (Enzyme Linked Immuno Sorbent Assay) 12. EMSA (Electrophoretic Mobility Shift Assay) 13. Gene Cloning 14. End labelling of DNA 15. DNA sequencing 16. RNA sequencing 17. Microarray 18. Generation of Genomic and cDNA library. 19. Western Blotting 20. Cell Culture 21. Fluorescent activated cell sorting (FACS) 22. DNA microarray 23. DNA foot printing 24. Chromatin Immunoprecipitation 25. Basics of Bioinformatics 26. Isolation of Proteins 27. Protein purification 28. Fluorescence In situ Hybridization