The Polymerase Chain Reaction

Methodology; manipulation of DNA, K. Hayashi; cloning PCR products, M. Frohman; multiplex PCRs, J.S. Chamberlain & J.R. Chamberlain; preparation of nucleic acids, D. Shibata; amplification of viral DNA and viral host cell mRNAs in situ, J. Embretson et al; quantitation - an overview, F. Ferre et al; quantification of DNAs by the polymerase chain using an internal control, D.M. Coen; RT-PCR and mRNA quantitation, J. Chelly and A. Kahn; analysis of T-cell repertoires by PCR, D. Spinella and J.M. Robertson; ultra-sensitive non-radioactive detection of PCR products - an overview, R.H. Tullis; fluorescent detection methods for PCR analysis, M.J. Heller; enzyme-labeled oligonucleotides, E. Tu & E. Jablonski; application of the hybridization protection assay to PCR, N.C. Nelson & S.H. McDonough; instrumentation - an overview, C. Oste; rapid cycle DNA amplification, C.T. Wittwer et al; automating PCR, H.R. Gardner; sequencing - an overview, R.A. Gibbs; and applications chapters on PCR and cancer, diagnostics, gene therapy, plant biology, forensics, and much more.