RNA-sequencing is one of the most powerful tools available to cancer researchers, but it is not without its challenges. In this book, Dr. Rathe provides a plethora of user-friendly tools and methods to assist cancer researchers in making the most of their RNA- sequencing data. Dr. Rathe describes a novel approach to prioritize the gene and pathway changes in controlled experiments (DEAPR), as well as a causal deconstruction process for uncovering disease-specific expression patterns in patient samples (NODC). In addition, her experience with characterizing unusual cellular changes directly related to the use of CRISPR and Sleeping Beauty is a must for researchers utilizing these cell modification techniques. Her personalized approach makes the reading experience enjoyable as well as informative. "I am pleased that Sue has pulled together this entertaining and easy-to-understand technical manual, so cancer researchers outside of my laboratory can benefit from her experience and avoid many of the pitfalls associated with RNA-seq data." -David A. Largaespada, PhD Professor, University of Minnesota