Regions have increasingly broken ground in the European sphere. For decades, they have been subject to forces both influencing them from above (regionalisation), as well as from below (regionalism). In the European Union, the regions and their actors have mainly manifested themselves via EU Regional Policy. Besides a closer look at the rationales behind this trend (e.g. multi-level governance) and the changes in the decision-making process, three case regions have thoroughly been addressed - Latvia, Scotland, and Saxony. This dissertation highlights the pitfalls, the possibilities, and the position of regions and its actors, in both the domestic and the European setting, in a clear and structured way. Dissertation. (Series: European Regions / Regionen in Europa - Vol. 4)