Curcumin are the main constituent of spices turmeric and is obtain from the rhizome of the East Indian plant Curcuma longa, family- Zingiberacae. It is one on the most promising natural products for both the biological and chemical study. Polyphenols have capacity to prevent and treatment of various disease due to their antioxidant capabilities. Polyphenols are derived from many components of the human food like turmeric. Curcumin has not only food derivatives it also shown multiple beneficial medicine properties to prevent the disease. The aim of this work was to develop and evaluate Curcumin loaded liposome and its bio- enhancement. Curcumin was selected as a natural drug for liposome formulation. Curcumin show variety of biological activity but it also shows poor bioavailability due to low aqueous solubility (1 ¿g/ml), poor absorption and rapid metabolism so that Piperine was selected as bio enhancer to improve curcumin bioavailability. Soy lecithin and cholesterol were used to prepared Curcumin and curcumin-piperine loaded liposome at different ratio by thin film hydration method because of easy to perform, and high encapsulation rates of lipid.