Fruit production has been the earliest of food sources meeting the needs of human race for centuries. Owing to varied climate under the sun, the fruit production has also got wide range in fruit types, nutritional values and production technologies. Citriculture has emerged as one of the major fruit production industries in the world. Like the other fruits, citrus is known for its versatility in use. It is used as fresh fruit, its peel is used for the extraction of essential oils, pulp in extraction of juices and also along with peel, used to prepare jams, jellies and marmalades. Owing to the ever increasing pressure from population explosion, the need has arisen to improve the crop to meet the demands which have a direct relation with galloping population of the global world of 21st century. In this research, I have been searching for an efficient procedure for callus induction, regenerative ability from different explants in Citrus reticulata Blanco, in order to provide a basis for genetic improvement. I have studied the effects of different growth regulators on callus initiation from different explants and shoot and root development from calli.