This paper synthesizes the existing literature about traditional and local ecological knowledge relating to biodiversity in Pacific Northwest forests in order to assess what is needed to apply this knowledge to forest biodiversity conservation efforts. We address four topics: (1) views and values people have relating to biodiversity, (2) the resource use and management practices of local forest users and their effects on biodiversity, (3) methods and models for integrating traditional and local ecological knowledge into biodiversity conservation on public and private lands, and (4) challenges to applying traditional and local ecological knowledge for biodiversity conservation. We focus on the ecological knowledge of three groups who inhabit the region: American Indians, family forest owners, and commercial nontimber forest product (NTFP) harvesters.