Transgenic Animal Technology: A Laboratory Handbook
Autor Carl A. Pinkerten Limba Engleză Hardback – 19 iun 2014
- Contains a comprehensive collection of transgenic animal and gene transfer methods
- Discusses background and introduction to techniques and animal systems
- Teaches practical step-by-step protocols
- Fully revised with updates to reflect state-of-the-art technology and associated changes to date
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Specificații
ISBN-13: 9780124104907
ISBN-10: 0124104908
Pagini: 714
Ilustrații: black & white illustrations
Dimensiuni: 152 x 229 x 38 mm
Greutate: 1.12 kg
Ediția:Revised
Editura: ELSEVIER SCIENCE
ISBN-10: 0124104908
Pagini: 714
Ilustrații: black & white illustrations
Dimensiuni: 152 x 229 x 38 mm
Greutate: 1.12 kg
Ediția:Revised
Editura: ELSEVIER SCIENCE
Public țintă
Students, postdoctoral fellows, and researchers at academic and industrial/agricultural laboratories using non-traditional transgenic models in such areas as molecular and cell biology, developmental biology, neuroscience, pharmacology, biophysiology, cardiology, oncology and genetics.Cuprins
Section OneOverview.Introduction to transgenic animals.Section TwoTransgenic animal production focusing on the mouse model.Transgenic animal production using DNA microinjection.Factors affecting transgenic animal production.Gene targeting in embryonic stem cells I: History and methodology.Gene targeting in embryonic stem cells II. Conditional technologies.Retrovirus-mediated gene transfer.Nuclear transfer technologies.Section ThreeProduction of transgenic laboratory and domestic animal species.Production of transgenic rats.Production of transgenic rabbits.Production of transgenic fish.Production of transgenic poultry.Production of transgenic swine by DNA Microinjection.Production of transgenic ruminants by DNA Microinjection.Production of transgenic non-human primates.Retrovirus-mediated gene transfer into oocytes.Section Four: Molecular biology, analyses and enabling technologiesVector design for transgene expression.Analysis of transgene integration.PCR optimization for detection of transgenic integration.Evaluation of transgene expression.Control of gene expression (from standard gain/loss of function to anti-sense to PNAs).Enabling technologies: Cryopreservation of gametes.Enabling technologies: Embryo culture considerations.Databases, Internet Resources and Genetic Nomenclature.
Recenzii
"...a valuable laboratory asset for any researcher working with genetically modified animals..." --Lab Animal
"...reasonably priced and provides a good review of transgenic technology that can be used by a novice or experienced Individual...figures and tables are of good quality and a nice supplement to the lnformation contained in the chapters." --Laboratory Animal Practitioner
"The handbook will be a valuable resource for two audiences, one seeking a broad appreciation for transgenic animal technology, which can be gained by reading the handbook cover to cover, and the other seeking concise descriptions of focused techniques, which can be found by selection of specific chapters." --Transgenics, 2013"This handbook would be valuable to the investigators producing transgenic animal models. It is well organized and the handbook provides a thorough description of current animal transgenic technology across a host of animals." --Analytical Biochemistry, 2003
"...reasonably priced and provides a good review of transgenic technology that can be used by a novice or experienced Individual...figures and tables are of good quality and a nice supplement to the lnformation contained in the chapters." --Laboratory Animal Practitioner
"The handbook will be a valuable resource for two audiences, one seeking a broad appreciation for transgenic animal technology, which can be gained by reading the handbook cover to cover, and the other seeking concise descriptions of focused techniques, which can be found by selection of specific chapters." --Transgenics, 2013"This handbook would be valuable to the investigators producing transgenic animal models. It is well organized and the handbook provides a thorough description of current animal transgenic technology across a host of animals." --Analytical Biochemistry, 2003