A laboratory study was conducted to elucidate the source-effect relationships for seven chemicals (sources) that can cause activated sludge process upset (effect). These chemicals were studied over a range of concentrations using both nitrifying and non-nitrifying laboratory-scale activated sludge sequencing batch reactors. Effects were characterized according to traditional methods of evaluating process effluent and mixed liquor quality. A range of process effects were observed for both biomass sources. Overall impact was assessed and the degree to which a chemical caused an impact on process performance was considered to be more detrimental than if a chemical had multiple process effects that were moderate. The order in which chemicals caused adverse effects for the nitrifying biomass was: ammonium