The present work is oriented to the chemical analysis of the seeds of Anadenathera peregrina or Piptadenia peregrina, commonly known as Yopo among the Amerindian communities and peoples of Southern Venezuela, specifically in Amazonas, and to determine its implications in Forensic Toxicology. Dimethyltryptamine, an alkaloid with hallucinogenic properties, was obtained, generating a pattern to be able to make comparisons in the field of Forensic Toxicology with blood samples and gastric content, through analytical methodologies such as ultraviolet spectrophotometry and thin layer chromatography. The result of the analysis is cold and pragmatic, not reflecting the administrative processes overcome to obtain the forensic samples, leaving a full experience of knowledge related to Forensic Medicine, Forensic Toxicology, Physical and Social Anthropology, Amazonian indigenous Cosmogony. Hoping that the reader will find in this writing a path that will guide him to his goals.