Cantitate/Preț
Produs

Analysis of Carbohydrates by Capillary Electrophoresis: CHROMATOGRAPHIA CE-Series, cartea 3

Autor Aran Paulus Editat de Kevin D. Altria Autor Antje Klockow-Beck
en Limba Engleză Paperback – 8 mar 2012
Carbohydrates are ubiquitous. They come as monomers, oligomers and polymers with a startling variety of chemical, physical and material properties. They affect almost every as­ pect of human live. For example they are an essential part of our nutrition. Cellulose, a car­ bohydrate polymer, acts as a central material in civilization: as building material for housing and as paper for written communication. Scientifically, the interest in carbohydrates ranges from structural elucidation of simple carbohydrates to fundamental biochemical processes such as photosynthesis and glycolysis. Always, a deeper understanding of a particular aspect of carbohydrate chemistry was accompanied by new methods of measuring and characteri­ zing this class of molecules. During the last decades a better understanding of the roles of carbohydrates in biochemi­ cal pathways developed. It turned out, that carbohydrates attached to proteins in glycoprote­ ins are involved in a number of molecular recognition and targeting processes. This lead to a new research field: Glycobiology. However, unlike for proteins or nucleic acids, there is at this time no consistent theory how chemical composition and structure controls function and inherent information. In order to develop the database, on which new theories and scientific hypothesis are founded, analytical methods to separate compounds with an enormous struc­ tural diversity and only minor differences in chemical or physical properties has to be deve­ loped, preferably using only minute quantities.
Citește tot Restrânge

Din seria CHROMATOGRAPHIA CE-Series

Preț: 37318 lei

Nou

Puncte Express: 560

Preț estimativ în valută:
7141 7489$ 5955£

Carte tipărită la comandă

Livrare economică 08-22 ianuarie 25

Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9783322850225
ISBN-10: 3322850226
Pagini: 196
Ilustrații: XIV, 176 p.
Dimensiuni: 170 x 240 x 10 mm
Greutate: 0.32 kg
Ediția:Softcover reprint of the original 1st ed. 1999
Editura: Vieweg+Teubner Verlag
Colecția Vieweg+Teubner Verlag
Seria CHROMATOGRAPHIA CE-Series

Locul publicării:Wiesbaden, Germany

Public țintă

Research

Cuprins

1 Introduction.- References.- 2 Capillary electrophoresis, instrumentation and modes.- 2.1 Electrophoretic mobility.- 2.2 Fused silica surface and electroosmotic flow.- 2.3 Plate number, migration time and resolution.- 2.4 Instrumentation.- 2.5 CE modes.- 3 Structures and properties of carbohydrates.- 3.1 Monosaccharides.- 3.2 Oligosaccharides.- 3.3 Polysaccharides.- 3.4 Glycoproteins.- 3.5 Glycosaminoglycans and Proteoglycans.- 3.6 Glycolipids.- 4 Separation and detection of carbohydrates in capillary electrophoresis.- 4.1 Separation and detection of non-derivatized carbohydrates.- 4.2 Separation and detection of derivatized carbohydrates.- 5 Applications.- 5.1 Mono- and disaccharides.- 5.2 Oligosaccharides.- 5.3 High molecular weight polysaccharides.- 5.4 Glycopeptides and Glycoproteins.- 5.5 Glycosaminoglycans.- 5.6 Glycolipids.- 5.7 Other glycoconjugates.

Notă biografică

Dr. Aran Paulus arbeitet und forscht im Bereich Analytik/Chromatographie für die Firma Soane in Kalifornien, USA. Dr. Antje Klockow-Beck ist in gleicher Funktion bei der Firma R.W. Johnson in Schaffhausen, Schweiz, tätig.

Textul de pe ultima copertă

Carbohydrates are next to nucleic acids and proteins the third important class of biopolymers and we are just beginning to understand their role in biological systems on a molecular level. In recent years, the post-translational modification of proteins has obtained increased interest. Specifically the glycosylation of enzymes, receptors and antibodies is the focus of numerous research porjects as it becomes clear that carbohydrates are involved in cellular communication and immuno recognition processes. In order ot understand this "sweet sugar language" and consequently use it in proteomics and the development of biopharmaceuticals, suitable analytical techniques have to be developed to separate carbohydrates with high selectivity in complex biological matrices and quantify them in in minute quantities.

The present monograph covers all aspects of carbohydrate analysis by Capillary Electrophoresis including instrumental aspects, derivatization for high sensitivity applications, separation conditions and applications in various fields and industries. It will be a valuable source of information for everyone involved in carbohydrate research in life sciences including glycobiology, biotechnology and immunology and in the food industry.

Caracteristici

Analytik für Praktiker in Forschung und Industrie