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Artificial DNA: Methods and Applications

Editat de Yury E. Khudyakov, Howard A. Fields
en Limba Engleză Hardback – 25 sep 2002
Combining elements of biochemistry, molecular biology, and immunology, artificial DNA can be employed in a number of scientific disciplines. Some of the varied applications include site-specific mutagenesis, hybridization, amplification, protein engineering, anti-sense technology, DNA vaccines, protein vaccines, recombinant antibodies, screening for genetic and pathogenic diseases, development of materials with new biochemical and structural properties, and many more.

Artificial DNA: Methods and Applications introduces the concept of artificial DNA that has been rationally designed and explains how it may be exploited in order to develop products that will achieve your intended purpose. The first part of the book covers methods of oligonucleotide synthesis and direct applications of synthetic DNA. The second part describes methods of gene assembly from synthetic oligonucleotides and applications of synthetic genes. The authors also discuss the different trends and future developments within each application area .

With state-of-the art research, the contributing authors describe how to engineer proteins using rational and semi-rational design to exhibit the desired traits and detail the various amplification reactions and hybridization techniques for modeling evolution and for use in basic research. The only text devoted to this subject, Artificial DNA offers a comprehensive review that allows you to understand the strategy, design, and applications of synthetic oligonucleotides.
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Specificații

ISBN-13: 9780849314261
ISBN-10: 0849314267
Pagini: 438
Ilustrații: 20 Halftones, black and white; 12 Tables, black and white; 7 Illustrations, color; 111 Illustrations, black and white
Dimensiuni: 178 x 254 x 29 mm
Greutate: 0.97 kg
Ediția:1
Editura: CRC Press
Colecția CRC Press

Public țintă

Professional Reference

Cuprins

Chemical Synthesis of Oligonucleotides: From Dreams to Automation. Artificial Hybridization Probes. Peptide Nucleic Acid (PNA): A DNA Mimic with a Pseudopeptide Backbone. Synthetic DNA in Amplification Reactions. Artificial Nucleic Acids and Genomic Profiling. Site-Directed Mutagenesis. Mimotopes. Artificial Genes for Chimeric Virus-like Particles. DNA Vaccines. Recombinant and Artificial Antigens as Diagnostic Reagents. Recombinant Antibodies.

Notă biografică

Yury E. Khudyakov, Howard A. Fields

Descriere

Introducing the concept of artificial DNA, this book presents state-of-the-art research on the process, trends, and application of artificial DNA. The text covers methods of oligonucleotide synthesis and direct uses of synthetic DNA, as well as methods of gene assembly from synthetic oligonucleotides and applications of synthetic genes. It explains how artificial DNA may be used to develop products that will achieve an intended purpose.Covering applications in synthetic DNA in various fields and industries, the book offers a comprehensive review that helps readers understand the strategy, design, and applications of synthetic oligonucleotides.