In this volume, the authors provide a wide range of themes dealing with the identification and application of biocatalysts. This includes various formats such as whole-cell or cell-free biocatalysis as well as immobilized variants. Along this line, the user may encounter various decision points and so guidance on the proper format for a desired application introduces the field and is supported by some advice on documentation for reproducible and fair data handling. Enzyme mining and screening can be done on various levels, too, and it does affect the later application chances as well. Respectively, selected examples for screening and biocatalyst application setups are given herein. Finally, it should be mentioned that processes need to be evaluated and biocatalysis is not always green per se! Hence, the authors hope to provide a supportive resource and some role models for the user to plan and succeed with a biocatalysis project.

• Guidance on how to identify, produce, describe and apply biocatalysts including documentation
• Broad perspective on biocatalysis including its advantages as well as hurdles
• Plethora of applications of a diverse set of enzyme-based catalysis