Biological and Biomedical Applications of Isoelectric Focusing: Biological Separations

1 Isoelectric Focusing of Human Saliva, Cerebrospinal Fluid, and Urine.- I. Introduction.- II. Isoelectric Focusing of Saliva.- III. Isoelectric Focusing of Cerebrospinal Fluid.- IV. Isoclectdc Focusing of Urine.- V. Conclusions.- VI.References.- 2 The Use of Gel in the Analysis of Hemoglobins.- I. Introduction.- II. Practical Applications.- III. Experimental Applications.- IV. References.- 3 Isoelectric Focusing of Allergens.- I. Introduction.- II. Methods.- III. Isoelectric Focusing of Allergens.- IV. Applications.- V. General Comments.- VI. References.- 4 Isoelectric Focusing of Muscle Proteins.- I. Introduction.- II. Technical Considerations.- III. Specific Applications.- IV. Conclusions and Predictions.- V. References.- 5 Studies of the Structure and Synthesis of Immunoglobulins by Isoelectric Focusing.- I. Introduction.- II. Methods of Isoelectric Focusing of Immunoglobulins.- III. Isoelectric Focusing of M-Proteins.- IV. Studies of Antibody Structure and Heterogeneity by Isoelectric Focusing.- V. Studies of Antibody Synthesis.- VI. References.- 6 The Use of Isoelectric Focusing in the Separation and Characterization of Nuclear Nonhistone Proteins.- I. Introduction.- II. General Properties of NHP.- III. Isoelectric Focusing of NHP.- IV. Artifacts and the Heterogeneity of NHP.- V. Conclusion.- VI. References.- 7 Isoelectric Focusing of Membrane Components.- I. Introduction.- II. Methods.- III. Biological Applications.- IV. References.- 8 Isoelectric Focusing of Seed Proteins.- I. Introduction.- II. Food Proteins.- III. Enzymes.- IV. Protease Inhibitors.- V. Lectins.- VI. Genetic Studies.- VII. Conclusion.- VIII. References.- 9 Isoelectric Focusing of Microbial Proteins.- I. Introduction.- II. Materials.- III. Methods.- IV. Isoelectric Focusing of Microbial Proteins.- V. Conclusions.- VI. References.- 10 Isoelectric Focusing of Serum Proteins.- I. Introduction.- II. Gel Isoelectric Focusing Alone.- III. Gel Electrofocusing Followed by Electrophoresis.- IV Individual Types of Protein in Serum or Plasma.- V. Clinical Applications.- VI. References.