Biophysical Chemistry of Proteins: An Introduction to Laboratory Methods

en Limba Engleză Hardback – 19 noi 2010

The book is structured in nine sections, each containing several chapters. The volume starts with an overview of analytical techniques and progresses through purification of proteins; protein modification and inactivation; protein size, shape, and structure; enzyme kinetics; protein-ligand interactions; industrial enzymology; and laboratory quality control. The book is targeted at all scientists interested in protein research.

Citește tot Restrânge

Toate formatele și edițiile

	Preț	Express
Paperback (1)	687^.82 lei 6-8 săpt.
Springer Us – 28 noi 2014	687^.82 lei 6-8 săpt.
Hardback (1)	647^.16 lei 6-8 săpt.
Springer Us – 19 noi 2010	647^.16 lei 6-8 săpt.

Preț: 647^.16 lei

Preț vechi: 761^.37 lei
-15% Nou

Puncte Express: 971

:
123^.85€ • 130^.26$ • 103^.17£

Carte tipărită la comandă

Livrare economică 03-17 ianuarie 25

Adaugă în coș

Wish list
Gift list
Am citit!

Adaugă în listă

Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781441972507
ISBN-10: 1441972501
Pagini: 528
Ilustrații: XVI, 510 p.
Dimensiuni: 210 x 279 x 28 mm
Greutate: 1.13 kg
Ediția:2011
Editura: Springer Us
Colecția Springer
Locul publicării:New York, NY, United States

Public țintă

Graduate

Cuprins

Analytical techniques. Microscopy. Single molecule techniques. Preparation of cells and tissues for microscopy. Principles of optical spectroscopy.Photometry. Fluorimetry. Chemiluminescence.Electrophoresis.Immunological methods.Isotope techniques.- Purification of proteins.Homogenisation and fractionisation of cells and tissues.Isolation of organelles. Precipitation methods.Chromatography. Membrane proteins. Determination of protein concentration.Cell culture.- Protein modification and inactivation. General technical remarks. Amine-reactive reagents. Thiol- and disulphide reactive reagents. Reagents for other groups. Cross-linkers.Detection methods. Spontaneous reactions in proteins.- Protein size and shape. Centrifugation.Osmotic pressure. Diffusion.Viscosity.Non-resonant interactions with electromagnetic waves.- Protein structure. Protein sequencing.Synthesis of peptides. Protein secondary structure. Structure of protein-ligand complexes. 3D-structures. Folding and unfolding of proteins.- Enzyme kinetics. Steady-state kinetics. Leaving the steady state: Analysis of progress curves.Reaction velocities. Isotope effects. Isotope exchange.- Protein-ligand interactions. General conditions for interpretable results. Binding equations.Methods to measure binding equilibria. Temperature effects on binding equilibrium and reaction rate.- Industrial enzymology. Industrial enzyme use.Immobilised enzymes.- Special statistics.Quality control. Testing whether or not a model fits the data.- Appendix. List of symbols.Greek alphabet. Properties of electrophoretic buffers. Bond properties. Acronyms.

Notă biografică

Dr. Buxbaum's research interests are enzymology and protein structure/function relationship. In addition, he has been teaching science and medical students in several countries. He is currently working as associate professor of biochemistry at Ross University School of Medicine.

Textul de pe ultima copertă

Undergraduate biochemistry courses cover what proteins do, as enzymes, receptors, hormones, motors or structural components. The much more interesting question is how can proteins achieve all these functions? Presented here is an overview of the methods used in such projects, their possible applications, and their limitations. Focusing on the biophysical chemistry of proteins, the text is accessible to those with a general background in chemistry, physics and mathematics, though a good understanding of protein structure and enzymology is required. The text may be used in courses of protein science, by students embarking on master- or PhD-thesis work in this area or by professionals who need a quick reminder about the essentials of a method.

Caracteristici

Focuses on the biophysical chemistry of proteins Presents an overview of the methods used in protein research Examines the possible applications and limitations of protein research

Papetărie, jocuri, reviste