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Blood'Brain Barrier: Biology and Research Protocols: Methods in Molecular Medicine, cartea 89

Editat de Sukriti Nag
en Limba Engleză Hardback – 6 aug 2003
Blood–brain barrier (BBB) breakdown leading to cerebral edema occurs in many brain diseases—such as trauma, stroke, inflammation, infection, and tumors—and is an important factor in the mortality arising from these con- tions. Despite the importance of the BBB in the pathogenesis of these diseases, the molecular mechanisms occurring at the BBB are not completely und- stood. In the last decade a number of molecules have been identified not only in endothelial cells, but also in astrocytes, pericytes, and the perivascular cells that interact with endothelium to maintain cerebral homeostasis. However, the precise cellular interactions at a molecular level in steady states and d- eases have still to be determined. The introduction of new research techniques during the last decade or so provide an opportunity to study the molecular mec- nisms occurring at the BBB in diseases. The Blood–Brain Barrier: Biology and Research Protocols provides the reader with details of selected morphologic, permeability, transport, in vitro, and molecular techniques for BBB studies, all written by experts in the field. Each part is preceded by a review that emphasizes the advantages and pitfalls of particular techniques, as well as offering much relevant current information. The techniques provided will be helpful to both beginners in BBB research and those more experienced investigators who wish to add a specific technique to those already available in their laboratories.
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Specificații

ISBN-13: 9781588290731
ISBN-10: 1588290735
Pagini: 564
Ilustrații: XIV, 550 p.
Dimensiuni: 178 x 254 x 40 mm
Greutate: 1.5 kg
Ediția:2003
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Medicine

Locul publicării:Totowa, NJ, United States

Public țintă

Research

Cuprins

Tissue Techniques.- Morphology and Molecular Properties of Cellular Components of Normal Cerebral Vessels.- Studies of Cerebral Vessels by Transmission Electron Microscopy and Morphometry.- Freeze-Fracture Studies of Cerebral Endothelial Tight Junctions.- Studies of Cerebral Endothelium by Scanning and High-Voltage Electron Microscopy.- Detection of Endothelial/Lymphocyte Interaction in Spinal Cord Microvasculature by Intravital Videomicroscopy.- Blood-Brain Barrier Permeability Techniques.- Pathophysiology of Blood-Brain Barrier Breakdown.- Pial Vessel Permeability to Tracers Using Cranial Windows.- Blood-Brain Barrier Permeability Using Tracers and Immunohistochemistry.- Ultracytochemical Studies of the Compromised Blood-Brain Barrier.- Immunogold Detection of Microvascular Proteins in the Compromised Blood-Brain Barrier.- Measurement of Blood-Brain and Blood-Tumor Barrier Permeabilities with [14C]-Labeled Tracers.- Blood-Brain Barrier Transport Techniques.- A Review of Blood-Brain Barrier Transport Techniques.- In Situ Brain Perfusion Technique.- Brain Efflux Index Method.- Transport Studies Using Membrane Vesicles.- Drug Transport Studies Using Quantitative Microdialysis.- Single-Pass Dual-Label Indicator Method.- Protein Transport in Cerebral Endothelium.- Transport Across the Choroid Plexuses In Vivo and In Vitro.- In Vitro Techniques.- An Overview of In Vitro Techniques for Blood-Brain Barrier Studies.- Isolation and Characterization of Human Brain Endothelial Cells.- Human Brain Microvessel Endothelial Cell and Leukocyte Interactions.- Development and Characterization of Immortalized Cerebral Endothelial Cell Lines.- Isolation and Characterization of Retinal Endothelial Cells.- Isolation and Characterization of Cerebral Microvascular Pericytes.- Molecular Techniques.- Molecular Biology of the Blood-Brain Barrier.- Blood-Brain Barrier Genomics.- Dynamic In Vitro Model of the Blood-Brain Barrier.- Phage Display Technology for Identifying Specific Antigens on Brain Endothelial Cells.- mRNA Detection in Cerebral Vessels by Nonradioactive In Situ Hybridization.- Proteomics of Brain Endothelium.- Immunoblot Detection of Brain Vascular Proteins.- Immunohistochemical Detection of Endothelial Proteins.- Genetically Altered Mice.- Cerebral Vascular Function in Genetically Altered Mice.- Generation of Transgenic Mice by Pronuclear Injection.- Mouse Genome Modification.

Recenzii

"An outstanding addition to the field! The book is unique in its organization and ease of reading and use as research resource. In my view, this book will emerge as one of the more cited and critical sources of information for all researchers exploring the nature of the blood-brain barrier."-Doody's Health Science Book Review Journal

Textul de pe ultima copertă

Despite the fact that the breakdown of the blood-brain barrier occurs in many brain diseases and is an important factor in mortality, the molecular mechanisms involved in the breakdown are not completely understood. In The Blood-Brain Barrier: Biology and Research Protocols, leading researchers and clinician-scientists present state-of-the-art morphologic, permeability, transport, in vitro, and molecular techniques to study the workings of the blood-brain barrier in health and disease. Described in step-by-step detail, each proven method includes an explanation of the theoretical basis of the method, a discussion of its advantages and limitations, notes on avoiding pitfalls, and examples of results obtained using the technique. At the cutting edge are techniques to explore the genomics and proteomics of brain endothelium.
Comprehensive and state-of-the-art, The Blood-Brain Barrier: Biology and Research Protocols offers pharmacologists, physiologists, neuroscientists, neurosurgeons, and neurologists an array of powerful tools to isolate undiscovered molecules, deepen our understanding of their role in health and disease, and identify targets for future gene therapy and therapeutic intervention.

Caracteristici

Includes supplementary material: sn.pub/extras