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Bone Marrow and Stem Cell Transplantation: Methods in Molecular Medicine, cartea 134

Editat de Meral Beksaç
en Limba Engleză Hardback – 3 mai 2007
Molecular Methods: Stem Cell Transplantation presents a compendium of cutting-edge research on the molecular steps involved in hematopoietic stem cell (HSC) activation and self-renewal. The emergence of HLA typing, and the positive impact it has had on the success of clinical transplantation is emphasized and discussed by notable stem cell researchers.
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Specificații

ISBN-13: 9781588295958
ISBN-10: 1588295958
Pagini: 244
Ilustrații: X, 234 p.
Dimensiuni: 152 x 229 x 19 mm
Greutate: 0.51 kg
Ediția:2007
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Medicine

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

Molecular Profiling of Hematopoietic Stem Cells.- Methods for Imaging Cell Fates in Hematopoiesis.- An Overview of HLA Typing for Hematopoietic Stem Cell Transplantation.- Sequence-Specific Primed PCR (PCR-SSP) Typing of HLA Class I and Class II Alleles.- HLA Typing with Sequence-Specific Oligonucleotide Primed PCR (PCR-SSO) and Use of the Luminex™ Technology.- Sequencing-Based Typing of HLA.- Molecular Typing Methods for Minor Histocompatibility Antigens.- Non-HLA Gene Polymorphisms in Stem Cell Transplantation.- Polymorphisms Within Epithelial Receptors.- Role of Natural Killer Cells and Killer Immunoglobulin-Like Receptor Polymorphisms.- Identification of Bone Marrow Derived Nonhematopoietic Cells by Double Labeling with Immunohistochemistry and In Situ Hybridization.- Molecular Methods Used for Detection of Minimal Residual Disease Following Hematopoietic Stem Cell Transplantation in Myeloid Disorders.- Molecular Methods Used for The Detection of Autologous Graft Contamination in Lymphoid Disorders.- Detection of Impending Graft Rejection and Relapse by Lineage-Specific Chimerism Analysis.- Application of Proteomics to Posttransplantational Follow-Up.

Caracteristici

Discussion of HLA typing, PCR-SSP typing, HLA antigens Chapters written by world-renown leaders in the field

Textul de pe ultima copertă

The second edition of Bone Marrow and Stem Cell Transplantation expands upon the previous edition with current, detailed methods on HLA, minor-HLA and Killer Immunoglobulin Like Receptor typing. With new chapters on immunophenotyping and functional characterization of stem cells are included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Bone Marrow and Stem Cell Transplantation, Second Edition serves as a guide in the application of molecular methods for routine or investigational purposes.