Calcium Regulating Hormones, Vitamin D Metabolites, and Cyclic AMP Assays and Their Clinical Application

1 Introduction.- 1.1 Clinical Application of Intact Parathyroid Hormone Determination.- 1.2 Clinical Use of Vitamin D Metabolite Assays (Calcidiol and Calcitriol).- 1.3 Diagnostic Value of the Peptides from the Calcitonin Gene.- 1.4 Immunoassay Vial for Improved Assay Performance.- 2 Parathyroid Hormone Assays and Clinical Application.- 2.1 Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 53–84) with Tyrosylated Peptide as Tracer.- 2.2 Sensitive Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 53–84) to Diagnose Hypoparathyroid Conditions: Guidelines for the Establishment of Highly Sensitive Assays.- 2.3 Rapid Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 53–84) as a Diagnostic Aid in Hypercalcemic Crisis.- 2.4 Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 44–68) with Tyrosylated Peptide as Tracer.- 2.5 Purification of 125Iodine-Labeled Tyrosylated Human Parathyroid Hormone Fragment (Residues 44–68), Optimized by Reverse-Phase High-Performance Liquid Chromatography.- 2.6 Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 28–48) with Tyrosylated Peptide as Tracer.- 2.7 Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 1–34) with Biotinylated Peptide as Tracer.- 2.8 Measurement of Intact Parathyroid Hormone by an Extracting Two-Site Immunoradiometric Assay.- 2.9 Measurement of Intact Parathyroid Hormone by a Two-Site Immunochemiluminometric Assay.- 2.10 A Sensitive Adenylate Cyclase Bioassay for Parathyroid Hormone.- 2.11 Immunological Properties of Asparagine versus Aspartic Acid at Residue 76 of Human Parathyroid Hormone (Residues 53–84).- 3 Parathyroid Hormone-Related Protein.- 3.1 Parathyroid Hormone-Related Protein.- 4 Cyclic AMP.- 4.1Plasma and Urinary Cyclic AMP Assay and Clinical Application.- 5 Vitamin D: Assays of 25-Hydroxyvitamin D (Calcidiol) and 1,25-Dihydroxyvitamin D (Calcitriol).- 5.1 Simultaneous Determination of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 by High-Performance Liquid Chromatography.- 5.2 Competitive Protein-Binding Assay for the Diagnosis of Hyper- and Hypovitaminosis D.- 5.3 A Competitive Protein-Binding Assay with Second Antibody Separation for the Diagnosis of Hyper- and Hypovitaminosis D.- 5.4 Interlaboratory Comparison of Calcidiol Determination.- 5.5 A Sensitive Radioimmunoassay for 1,25-Dihydroxyvitamin D3 (Calcitriol) after High Performance Liquid Chromatography of Plasma or Serum Extracts.- 5.6 A Double Antibody Radioimmunoassay for measurement of 1?, 25-Dihydroxyvitamin D3.- 5.7 Competitive Protein-Binding Assay of Calcitriol with an Advanced Preparation of Bovine Calf Thymus Cytosol.- 6 Assay of Calcitonin and Related Peptides.- 6.1 Design of New Methods for Measuring Calcitonin and Related Peptides Using Monoclonal Antipeptide Antibodies.