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Cell-Based Microarrays: Review of Applications, Developments and Technological Advances de Ella Palmer

Cell-Based Microarrays: Review of Applications, Developments and Technological Advances: SpringerBriefs in Cell Biology

Autor Ella Palmer
en Limba Engleză Paperback – 2 apr 2014
This book is a review on the evolution of cell-based microarrays and an update to the author's earlier book Methods in Molecular Biology: Cell-Based Microarrays. Since their development in 2001, cell-based microarrays have advanced significantly to include expression arrays, short interfering RNA arrays and antibody arrays. The surface used to coat the glass slides has also been significantly improved to allow non-adherent cells to bind to the arrays.
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Specificații

ISBN-13: 9781493905935
ISBN-10: 1493905937
Pagini: 80
Ilustrații: X, 100 p. 14 illus., 13 illus. in color.
Dimensiuni: 155 x 235 x 7 mm
Greutate: 0.25 kg
Ediția:2014
Editura: Springer
Colecția Springer
Seria SpringerBriefs in Cell Biology

Locul publicării:New York, NY, United States

Public țintă

Research

Cuprins

1 Cell-based microarrays: recent advances for gene function analyses.- 2 Review of siRNA/shRNA Applications in Cell-Based Microarrays.- 3 Antibody microarrays for cell-based assays: The use of micro-arrayed antibodies for exploring cell surface diversity or whole cell functionality.- 4 Polymer microarrays for high throughput biomaterials discovery.- 5 Microfluidics cell-based microarray review.

Notă biografică

Ella Palmer is a molecular biology expert. She undertook her Ph.D. at the University of Cambridge, where she developed a large-scale cell-based microarray and identified potential new genes involved in apoptosis, for which a patent was registered. She has since held postdoctoral positions at Imperial College in London, examining the cell polarity of Drosophila photoreceptors, and at University College London, working on a quantitative systems biology initiative for single cell analysis of the NFAT pathway. Most recently she has been working as an intern at a medical writing company.

Caracteristici

Explores the evolution of cell-based microarrays Reviews and updates four key aspects of cell-based microarrays Updates the earlier work Methods in Molecular Biology: Cell-Based Microarrays

Textul de pe ultima copertă

As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.