Cell Line Development: Cell Engineering, cartea 6
Editat de Mohamed Al-Rubeaien Limba Engleză Paperback – 14 mar 2012
This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.
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Specificații
ISBN-13: 9789400730403
ISBN-10: 9400730403
Pagini: 264
Ilustrații: VIII, 253 p.
Dimensiuni: 155 x 235 x 14 mm
Greutate: 0.37 kg
Ediția:2009
Editura: SPRINGER NETHERLANDS
Colecția Springer
Seria Cell Engineering
Locul publicării:Dordrecht, Netherlands
ISBN-10: 9400730403
Pagini: 264
Ilustrații: VIII, 253 p.
Dimensiuni: 155 x 235 x 14 mm
Greutate: 0.37 kg
Ediția:2009
Editura: SPRINGER NETHERLANDS
Colecția Springer
Seria Cell Engineering
Locul publicării:Dordrecht, Netherlands
Public țintă
ResearchCuprins
Use of MAR Elements to Increase the Production of Recombinant Proteins.- Expression Engineering – The IE2 Promoter/Enhancer from Mouse CMV.- Defeating Randomness – Targeted Integration as a Boost for Biotechnology.- Importance of Genetic Environment for Recombinant Gene Expression.- Expression Vector Engineering for Recombinant Protein Production.- Cell Xpress™ Applications in Development and Characterization of Biopharmaceutical Recombinant Protein Producing Cell Lines.- Selection Methods for High-Producing Mammalian Cell Lines.- Engineering Mammalian Cells for Recombinant Monoclonal Antibody Production.- Engineering Cell Function by RNA Interference.- Apoptosis and Autophagy Cell Engineering.- Glycoengineering and Modeling of Protein N-Glycosylation.- Engineering the Secretory Pathway in Mammalian Cells.
Caracteristici
Presents an overall picture of the current state of knowledge in Cell Line Development Designed to offer a state-of-the-art review with in–depth assessments and perspectives of expression engineering through understanding the molecular and cellular basis of cell culture systems The various chapters are describing the necessary methodologies for using cell engineering tools for the improvement of cell culture processes Describes the most recent methods used to establish mammalian host cell lines and expression systems, optimisation of selection systems to reduce development times and improvement of glycosylation and secretion