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Contemporary Approaches to the Study of Pain: From Molecules to Neural Networks: Neuromethods, cartea 178

Editat de Rebecca P. Seal
en Limba Engleză Paperback – 27 mai 2023
This volume contains experimental approaches that are currently revolutionizing our understanding of the neurobiology of pain. The chapters cover many cutting-edge methods including the identification of gene expression profiles, transcriptomes or translatomes, from individual cells or defined groups of cells in rodents and primates;  the electrophysiological investigation of human tissues, such as human dorsal root ganglion neurons; ways to assess modality response profiles of neurons using calcium imaging in vitro and in vivo; and somatosensory behaviors in rodents using high-speed videography and machine learning.  In the Neuromethods series style, the chapters include detailed advice from specialists to obtain successful results in your laboratory.

Cutting-edge and comprehensive, Contemporary Approaches to the Study of Pain: From Molecules to Neural Networks is a valuable resource for scientists and researchers interested in making impactful contributions to our understanding of pain.

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Specificații

ISBN-13: 9781071620410
ISBN-10: 107162041X
Ilustrații: XIII, 458 p. 76 illus.
Dimensiuni: 178 x 254 mm
Greutate: 0.82 kg
Ediția:1st ed. 2022
Editura: Springer Us
Colecția Humana
Seria Neuromethods

Locul publicării:New York, NY, United States

Cuprins

Single-Cell RNA-Sequencing of Somatosensory Neurons.- Preparation of Human and Rodent Spinal Cord Nuclei for Single-Nucleus Transcriptomic Analysis.- Multiplex In Situ Hybridization of the Primate and Rodent DRG and Spinal Cord.- Using Translating Ribosome Affinity Purification (TRAP) to Understand Cell-Specific Translatomes in Pain States.- Ex Vivo Skin-Teased Fiber Recordings from Tibial Nerve.- Single Unit Electrophysiological Recordings of Primary Muscle Sensory Neurons using a Novel Ex Vivo Preparation.- Electrophysiological Recording Techniques from Human Dorsal Root Ganglion.- Human Pluripotent Stem Cell-Derived Sensory Neurons: A New Translational Approach to Study Mechanisms of Sensitization?.- Intraspinal Transplantation of Precursors of Cortical GABAergic Interneurons to Treat Neuropathic Pain.- A Co-Culture System for Studying Dorsal Spinal Cord Synaptogenesis.- Visualizing Synaptic Connectivity using Confocal and Electron Microscopy: Neuroanatomical Approaches to Define Spinal Circuits.- Using Viral Vectors to Visualize Pain-Related Neural Circuits in Mice.- Recording Pain-Related Brain Activity in Behaving Animals using Calcium Imaging and Miniature Microscopes.- Optical Imaging of the Spinal Cord for the Study of Pain: From Molecules to Neural Networks.- In Vivo Calcium Imaging of Peripheral Ganglia.- Optogenetic Modulation of the Visceromotor Response to Reveal Visceral Pain Mechanisms.- Use of Optogenetics for the Study of Skin-Nerve Communication.- Channelrhodopsin-2 Assisted Circuit Mapping in the Spinal Cord Dorsal Horn.- Production of AAVs and Injection into the Spinal Cord.- The Use of Intraspinally Injected Chemogenetic Receptor, PSAM-GlyR, to Probe the Functional Role of Spinal Dorsal Horn Neurons.- Measuring Mouse Somatosensory Reflexive Behaviors with High-Speed Videography, Statistical Modeling, and Machine Learning.


Textul de pe ultima copertă

This volume contains experimental approaches that are currently revolutionizing our understanding of the neurobiology of pain. The chapters cover many cutting-edge methods including the identification of gene expression profiles, transcriptomes or translatomes, from individual cells or defined groups of cells in rodents and primates;  the electrophysiological investigation of human tissues, such as human dorsal root ganglion neurons; ways to assess modality response profiles of neurons using calcium imaging in vitro and in vivo; and somatosensory behaviors in rodents using high-speed videography and machine learning.  In the Neuromethods series style, the chapters include detailed advice from specialists to obtain successful results in your laboratory.

Cutting-edge and comprehensive, Contemporary Approaches to the Study of Pain: From Molecules to Neural Networks is a valuable resource for scientists and researchers interested in making impactful contributions to our understanding of pain.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts