Cooperativity and Regulation in Biochemical Processes

1 Introducing the Fundamental Concepts.- 2 The Binding Isotherm.- 3 Adsorption on a Single-Site Polymer with Conformational Changes Induced by the Binding Process.- 4 Two-Site Systems: Direct and Indirect Cooperativity.- 5 Three-Site Systems: Nonadditivity and Long-Range Correlations.- 6 Four-Site Systems: Hemoglobin.- 7 Large Linear Systems of Binding Sites.- 8 Regulatory Enzymes.- 9 Solvent Effects on Cooperativity.- Appendices.- A. Pair and Triplet Correlations between Events.- B. Localization of the Adsorbent Molecules and Its Effect on the Binding Isotherm.- C. Transition from Microstates to Macrostates.- D. First-Order Correction to Nonideality of the Ligand’s Reservoir.- E. Relative Slopes of Equilibrated and “Frozen-in” BIs in a Multimacrostate System.- F. Spurious Cooperativity in Single-Site Systems.- G. The Relation between the Binding Isotherm and the Titration Curve for Two-Site Systems.- H. Fitting Synthetic Data.- I. A Comment on the Nomenclature.- J. Average Binding Constants and Correlation Functions.- K. Utility Function in a Binding System.- Abbreviations Used in the Text.- References.