This book constitutes the refereed proceedings of the 12th International Conference on Digital Forensics and Cyber Crime, ICDF2C 2021, held in Singapore in December 2021. Due to COVID-19 pandemic the conference was held virtually. The 22 reviewed full papers were selected from 52 submissions and present digital forensic technologies and techniques for a variety of applications in criminal investigations, incident response and information security. The focus of ICDS2C 2021 was on various applications and digital evidence and forensics beyond traditional cybercrime investigations and litigation.