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DNA and RNA Isolation Techniques for Non-Experts: Techniques in Life Science and Biomedicine for the Non-Expert

Autor Akash Gautam
en Limba Engleză Paperback – 31 mar 2023
This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.
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Specificații

ISBN-13: 9783030942328
ISBN-10: 3030942325
Pagini: 203
Ilustrații: XVI, 203 p. 58 illus., 48 illus. in color.
Dimensiuni: 155 x 235 x 15 mm
Greutate: 0.31 kg
Ediția:1st ed. 2022
Editura: Springer International Publishing
Colecția Springer
Seria Techniques in Life Science and Biomedicine for the Non-Expert

Locul publicării:Cham, Switzerland

Cuprins

1-Basic Idea About Nucleic Acids For Non-Experts.- 2-General Steps During Isolation Of DNA And RNA.- 3-Phenol-Chloroform DNA Isolation Method.- 4-RNA Isolation By The Guanidinium-Acid-Phenol Method.- 5-Spin Column-Based Isolation Of Nucleic Acid.- 6-Isolation Of Plasmid DNA By Alkaline Lysis.- 7-DNA Isolation By Hydrophilic Ionic Liquid Treatment.- 8-Lithium Chloride Based Isolation Of DNA And RNA.- 9-Isolation Of Bacteriophage DNA By Peg Method.- 10-DNA Isolation By Chelex Method.- 11-DNA Isolation By Lysozyme And Proteinase K.- 12-Salting-Out Method For Blood Samples.- 13-CTAB Or SDA Based Isolation Of Plant’s DNA.- 14-DNA Extraction By Spooling Method.- 15-Magnetic Bead-Based Nucleic Acid Isolation.- 16-Density Gradient-Based Nucleic Acid Isolation.- 17-DNA Extraction From Agarose Gel Though Paper-Strips.- 18-Transformation Or Genetic Modification Of Cells/Organisms.- 19-Gene Cloning And Vectors.- 20-Polymerase Chain Reaction (PCR).- 21-Southern And Northern Blotting.- 22-Genome Mapping.- 23-Applications Of DNA Sequencing Technologies For Current Research.

Notă biografică

Dr. Akash Gautam is an Assistant Professor at the Center for Neural and Cognitive Sciences, University of Hyderabad. Dr. Gautam is currently serving as a section editor for the Encyclopedia of Animal Cognition and Behavior (Springer Nature c. 2020).

Textul de pe ultima copertă

This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.

Caracteristici

Accessible guide for junior researchers and those in adjacent fields beginning interdisciplinary research Presents basic concepts, step-by-step instructions, and abundant illustrations Addresses the benefits and limitations of each technique, and troubleshoots common issues