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E. coli: Shiga Toxin Methods and Protocols de Dana Philpott

E. coli: Shiga Toxin Methods and Protocols: Methods in Molecular Medicine, cartea 73

Editat de Dana Philpott, Frank Ebel
en Limba Engleză Paperback – 8 oct 2011
The study of the pathogenesis of Shiga toxin-producing Escherichia coli (STEC) infections encompasses many different disciplines, including clinical microbiology, diagnostics, animal ecology, and food safety, as well as the cellular microbiology of both bacterial pathogenesis and the mechanisms of toxin action. E. coli: Shiga Toxin Methods and Protocols aims to bring together a number of experts from each of these varied fields in order to o- line some of the basic protocols for the diagnosis and study of STEC pat- genesis. We hope that our book will prove a valuable resource for the clinical microbiologist as well as the cellular microbiologist. For the clinical microbiologist, our aim is to detail a number of current protocols for the detection of STEC in patient samples, each of which have their own advantages. Chapter 1 provides an introduction into the medical significance of STEC infections. Chapters 2–7 follow with protocols for the diagnosis and detection of STEC bacteria in patient and animal samples.
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Specificații

ISBN-13: 9781617372643
ISBN-10: 1617372641
Pagini: 364
Ilustrații: XII, 352 p.
Dimensiuni: 152 x 229 x 19 mm
Greutate: 0.49 kg
Ediția:2003
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Medicine

Locul publicării:Totowa, NJ, United States

Public țintă

Research

Cuprins

The Medical Significance of Shiga Toxin-Producing Escherichia coli Infections.- Methods for Detection of STEC in Humans.- Serological Methods for the Detection of STEC Infections.- Detection and Characterization of STEC in Stool Samples Using PCR.- Molecular Typing Methods for STEC.- STEC in the Food Chain.- STEC as a Veterinary Problem.- Cellular Microbiology of STEC Infections.- Analysis of Pathogenicity Islands of STEC.- Generation of Isogenic Deletion Mutants of STEC.- Generation of Monoclonal Antibodies Against Secreted Proteins of STEC.- Microscopic Methods to Study STEC.- Detection and Characterization of EHEC-Hemolysin.- Shiga Toxin Receptor Glycolipid Binding.- Methods for the Purification of Shiga Toxin 1.- Methods for the Identification of Host Receptors for Shiga Toxin.- Shiga Toxin B-Subunit as a Tool to Study Retrograde Transport.- Measuring pH Within the Golgi Complex and Endoplasmic Reticulum Using Shiga Toxin.- Detection of Shiga Toxin-Mediated Programmed Cell Death and Delineation of Death-Signaling Pathways.- Interaction of Shiga Toxin with Endothelial Cells.- Shiga Toxin Interactions with the Intestinal Epithelium.- Protocols to Study Effects of Shiga Toxin on Mononuclear Leukocytes.- Animal Models for STEC-Mediated Disease.- Gnotobiotic Piglets as an Animal Model for Oral Infection with O157 and Non-O157 Serotypes of STEC.- Bovine Escherichia coli O157:H7 Infection Model.

Recenzii

"The authors should be thanked for providing this valuable resource tool that is highly recommended to microbiologists, molecular biologists and to all who are interested in bacterial toxin action. All medical libraries should house a copy of this text. The book also will serve as an excellent teaching guide for students and fellows particularly in postgraduate programs." -Folia Microbiologica

Textul de pe ultima copertă

Shiga toxin-producing Escherichia coli (STEC) and its isolated cytotoxin (Stx), also known as "hamburger E. coli" are continuing sources of significant-and sometime fatal-human and animal infections. In E. coli: Shiga Toxin Methods and Protocols, a multidisciplinary panel of leading experimentalists details the key protocols for the diagnosis and study of STEC and Stx pathogenesis. Described in step-by-step detail, these readily reproducible techniques range from those for the diagnosis and detection of STEC bacteria in patient and animal samples, to those for studying the cellular microbiology of STEC infections, especially host-pathogen interactions and the hemolysin of STEC. There are also protocols for studying the details of Shiga toxin (Stx) biology-from the purification of the toxin to the effects of Stx on various host cell functions-and for exploring STEC-mediated disease in various animal models. Each protocol has been thoroughly tested and optimized to produce robust and successful experimental results, and includes troubleshooting tips, advice on avoiding pitfalls, and alternate methods.
Comprehensive and up-to-date, E. coli: Shiga Toxin Methods and Protocols offers bacteriologists and medical microbiologists today's most powerful tools for illuminating the molecular and cellular microbiology of STEC's bacterial pathogenesis, as well as the mechanisms of its toxicity.

Caracteristici

Includes supplementary material: sn.pub/extras