Enzyme Dynamics and Regulation de P. Boon Chock

Enzyme Dynamics and Regulation

P. Boon Chock

Charles Y. Huang

C. L. Tsou

Jerry H. Wang

en Limba Engleză Paperback – 13 oct 2011

Recent developments in concepts and techniques have brought enzyme research to a changing yet exciting stage. Enzymes have served as indispensable tools in the phenomenal rise of molecular biology, and the resultant biotechnology thrusts enzymes to new heights and territories. This volume, the proceedings of a recent symposium on the Dynamics of Soluble and Immobilized Enzyme Systems, provides a current overview of the field to help scientists utilize long-established and newly acquired information.

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Cuprins

Enzyme Kinetics and Mechanisms.- 1 Mechanistic Studies on DNA Polymerase I.- 2 Assembly and Catalytic Functions of the Subunits of Succinyl Coenzyme A Synthetase.- 3 Fatty Acid Synthetase of Chicken Liver: A Novel Active-Site Structure for Condensation Comprised of SH Groups from a Cysteine Residue and an Oscillating Phosphopantetheine Swinging Arm on Adjacent Subunits.- 4 Regulatory Properties of Glucokinase.- 5 Mechanism of Activation of Calmodulin-Dependent Phosphatase by Divalent Metal Ions.- 6 Studies on the Mechanism and Molecular Mode of Regulation of Fructose-1,6-bisphosphatase.- 7 Phosphorylation and dAMP Inhibition of Snake Muscle Fructose-1,6-bisphosphatase.- 8 Identification and Characterization of Intermediates in the Mechanism of Enzyme Action.- 9 Conformational Dynamics in RNA-Protein Interactions: Immobilization of the Functional Domains in tRNAfMet and Methionyl-tRNA Synthetase.- 10 Structural and Kinetic Studies of E. coli Glutamine Synthetase.- 11 Cu, Zn Superoxide Dismutase: A Case of Metalloenzyme Catalysis in Which the Protein Moiety Plays a Major Role.- Regulatory Enzymes.- 12 Dynamic Participation of Protein Domains in Catalysis by 2-Oxo Acid Dehydrogenase Multienzyme Complexes.- 13 Membrane-Bound GTP-Transducin Efficiently Activates Retinal cGMP Phosphodiesterase.- 14 Signal Transduction in the ?-Adrenoceptor-Dependent Adenylate Cyclase.- 15 Acetyl-CoA Carboxylase: Correlation of Phosphorylation State with Allosteric Properties and Physiological State.- 16 Role of Cyclic Cascades in Metabolic Regulation.- 17 Regulation of Glutamine Synthetase Activity and Its Biosynthesis in Escherichia coli: Mediation by Three Cycles of Covalent Modification.- 18 Regulation of ATP, Mg-Dependent Protein Phosphatases.- 19 Molecular Mechanisms of AllostericRegulation in Aspartate Transcarbamylase.- 20 Dynamic Interactions of the Second Messenger Systems.- 21 Role of Multienzyme Complexes in the Integration of Cellular Metabolism.- Enzyme-Mediated Processes.- 22 Dynamics of Deoxynucleotide Synthesis in Relation to DNA Replication.- 23 Differential Stabilization of Left-Handed Z-DNA and Z-RNA In Vitro and In Vivo.- 24 Interaction of Restriction Endonucleases with Phosphorothioate DNA.- 25 Role of Conformational Dynamics of Myosin in Muscle Contraction.- 26 Enzymatic Modulation of Cytoskeletal Self-Assembly: ADP Ribosylation of Microtubule Protein Components.- 27 Human Angiogenin: An Organogenic Protein.- Membrane-Bound and Immobilized Enzymes.- 28 Structure and Mechanism of Action of a Membrane-Bound Enzyme: Chloroplast Coupling Factor.- 29 Electrostatic Effects and the Dynamics of Multienzyme Reactions at the Surface of Plant Cells.- 30 Energy Transduction by Electroconformational Coupling.- 31 Topics in Petroleum Biotechnology.- Enzyme Methodology.- 32 Experimental Determination of Rate Constants in Enzymatic Reactions.- 33 Applications of Alternative Substrate Kinetics: In Vivo and In Vitro Data on Aminoglycoside Antibiotic Inactivating Enzymes.- 34 Kinetics of Irreversible Modification of Enzyme Activity.- 35 Applications of Stable Isotopes in Biochemistry.- 36 Enzyme Dynamics in Nonaqueous Media at Subzero Temperatures.- 37 Kinetic-Structural Organization of Enzyme Systems.- Enzyme Structure and Functions.- 38 Relation Between Structure and Function in cAMP-Dependent Protein Kinases.- 39 Conformation and Dynamics of Oligomeric Enzymes.- 40 Coenzyme Binding Site of NAD-Dependent Isocitrate Dehydrogenase.- 41 Significance of Domain Structure of Calmodulin on the Activation of Ca2+-Calmodulin Requiring Enzymes.- 42 Modification of Leucyl-tRNA Synthetase by Affinity Labeling and Limited Proteolysis.- 43 NAD Metabolism in Eukaryotic Cells: Purification and Characterization of NMN Adenylyltransferase from Baker’s Yeast.- 44 Specific ?-d-Fucosidase from Aspergillus phoenicis.- Site-Directed Mutagenesis of Enzymes.- 45 Formation of Active Aspartate Transcarbamoylase from Defective Polypeptide Chains Produced by Site-Directed Mutagenesis.- 46 Catalytic Mechanisms Revealed by Protein Engineering of Tyrosyl-tRNA Synthetase.- 47 Site-Directed Mutagenesis of Alkaline Phosphatase.