This book by Dr. Nikolai Vekshin is the 2-nd edition (with additions and corrections). It is a brief course in fluorescence spectroscopy of biomacromolecules and their complexes. General items on spectroscopy, engineering of experiments and biological examples are given. Proteins, enzymes, DNA, oligonucleotides, biomembranes, coenzymes, antibiotics, fluorescent probes, dyes etc. are presented as examples (basically from own experience of the author). The book is intended for biophysicists, biochemists, molecular biologists, physical-chemists and other researchers, interested in use of fluorescent methods.