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Functional Nucleic Acids for Analytical Applications: Integrated Analytical Systems

Editat de Yingfu Li, Yi Lu
en Limba Engleză Hardback – 12 dec 2008
Nature has long used nucleic acid aptamers and enzymes for regulatory activities, such as the recently discovered “riboswitches” involved in gene expression. The existence of a large array of natural and artificial functional nucleic acids has generated tremendous enthusiasm and new opportunities for molecular scientists from diverse disciplines to devise new concepts and real applications that take advantage of those nucleic acids for sensing and other analytical applications. This book provides a timely and comprehensive overview of recent advances in the field, from leading experts in biology, chemistry, and engineering. A variety of topics are covered, from fundamentals of functional nucleic acids, to their applications as sensors, to nanotechnologies; as well as integration of functional nucleic acids into practical analytical systems.
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Specificații

ISBN-13: 9780387737102
ISBN-10: 0387737103
Pagini: 396
Ilustrații: VIII, 396 p.
Dimensiuni: 155 x 235 x 24 mm
Greutate: 0.74 kg
Ediția:2009
Editura: Springer
Colecția Springer
Seria Integrated Analytical Systems

Locul publicării:New York, NY, United States

Public țintă

Research

Cuprins

Overview of Functional Nucleic Acids and Their Analytical Applications.- Introductory Remarks.- Natural Functional Nucleic Acids: Ribozymes and Riboswitches.- Artificial Functional Nucleic Acids: Aptamers, Ribozymes, and Deoxyribozymes Identified by In Vitro Selection.- Functional Nucleic Acid Sensors Based on Different Transduction Principles.- Fluorescent Aptamer Sensors.- Fluorescent Ribozyme and Deoxyribozyme Sensors.- Colorimetric and Fluorescent Biosensors Based on Directed Assembly of Nanomaterials with Functional DNA.- Electrochemical Approaches to Aptamer-Based Sensing.- Amplified DNA Biosensors.- Other Emerging Analytical Applications.- Aptamers in Affinity Separations:Capillary Electrophoresis.- Aptamers in Affinity Separations: Stationary Separation.- Aptamer Microarrays.- The Use of Functional Nucleic Acids in Solid-Phase Fluorimetric Assays.- Functional Nucleic Acid Sensors as Screening Tools.- Nucleic Acids for Computation.- DNAzymes in DNA Nanomachines and DNA Analysis.

Recenzii

From the reviews:
"This book consists of 15 chapters contributed by experts in the field of functional nucleic acids, mostly in the area of specific analytical applications of nucleic acids … . this book affords a comprehensive collection of clearly presented, up-to-date reviews that broadly cover the numerous applications of functional nucleic acids. … The chapters are of high quality … that will be useful to newcomers entering the field of functional nucleic acids and to those interested in exploring other applications complementary to their own niches." (Jennifer S. Brodbelt, Journal of the American Chemical Society, Vol. 131 (32), 2009)
“The book is well structured and combines 15 insightful reviews written by many leading experts. … Functional Nucleic Acids for Analytical Applications is a comprehensive and well written book. With its excellent review of recent progress and critical outlook of future directions it will be very valuable for researchers wishing to explore the unique properties of FNAs for different applications in analytical and bioanalytical chemistry.”­­­ (Xiaohong Fang, Analytical and Bioanalytical Chemistry, Vol. 398, June, 2010)

Textul de pe ultima copertă

Both DNA and RNA can serve purposes beyond simple genetic storage and transfer – for example, they can also be employed as enzymes (for catalysis) and receptors (for ligand binding). Increasingly, researchers are making interesting use of these molecules, now collectively called functional nucleic acids. This book, containing chapters written by leading experts in relevant fields, provides timely accounts of diverse analytical applications that take advantage of the functional nucleic acids. The book is written for academic, clinical and industrial scientists and graduate students who may consider exploring functional nucleic acids in their research or practice.

Caracteristici

First in the rapidly emerging field on analytical applications for nucleic acids With interdisciplinary approach, a valuable tool for researchers in chemistry, biology, and engineering One of the first books in the series Integrated Microanalytical Systems, recently renamed Integrated Analytical Systems, edited by R. Potyrailo