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Modern Clinical Molecular Techniques

Editat de Peter Hu, Madhuri Hegde, Patrick Alan Lennon
en Limba Engleză Hardback – 13 mai 2012
This timely book covers the need to know clinical practices for all those involved in molecular laboratory science. The field of molecular medicine is evolving at an astounding speed. Propelled by the new insights and technologies, advances are being made at an unprecedented rate. With dual measure given to today’s breakthroughs, this book is a collection of the most current practices relevant to the clinical molecular laboratorian. It begins with an introductory section on techniques and procedure.  It then presents four separate sections on infectious disease, oncology, pre/post-natal, and identity testing, with specific chapters clearly outlining clinical protocols used in daily practice.
Modern Clinical Molecular Techniques cuts to the heart of what is essential for the practicing molecular laboratory scientist. It is an outstanding resource for those operating within or looking to set up a clinical molecular laboratory.
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Specificații

ISBN-13: 9781461421696
ISBN-10: 1461421691
Pagini: 452
Ilustrații: XIII, 436 p.
Dimensiuni: 178 x 254 x 28 mm
Greutate: 0.98 kg
Ediția:2012
Editura: Springer
Colecția Springer
Locul publicării:New York, NY, United States

Public țintă

Professional/practitioner

Cuprins

Preface
Acknowledgement
Contributors
 
 
Section I   Current Molecular Laboratory Methods
 
1        Specimen Collection, Handling and Processing
Lindsy Hengesbach and John Gerlach
 
2        DNA/RNA Isolation and Quantitation
Jing Wang
 
3        Test Validation
Cindy McCloskey and S Terence Dunn
 
4        In Vitro Amplification Methods in Molecular Diagnostics
C. Alexander Valencia and Bradford Coffee
 
5        Whole Genome Sequencing in the Clinical Laboratory
Tina Hambuch, Brad Sickler, Arnold Liao, Suneer Jain, and Philip D. Cotter
 
6        Estimation of Exon Dosage Using Real-Time Quantitative Polymerase Chain Reaction
Juliana Purrazzella and Arupa Ganguly
 
7        Reporting Clinical Molecular Genetic Laboratory Results
Victor Wei Zhang and Lora J. H. Bean
 
 
Section II Clinical Molecular Infectious Disease Testing
 
8        HIV-1 Genotyping and Virtual Phenotyping
Fangling Xu and Jianli Dong
 
9        COBAS® Ampliprep/Taqman HIV-1 Test
Tavat A. Buraruk and Charles E. Stager
 
10    Hepatitis C Virus (HCV) Genotype Assay (LiPA)
Charles E. Stager and Tavat A. Buraruk
 
11    COBAS® Ampliprep/Taqman HCV Test
Charles E. Stager and Tavat A. Buraruk
 
12    Epstein-Barr Virus and Cytomegalovirus Viral Load Monitoring by Quantitative Real-Time Polymerase Chain Reaction
Su S. Chen, Seema Hai, and Rajyalakshmi Luthra
 
13    Quantification of BK Viral Load
Hai Wu and Jianli Dong
 
14    Molecular Diagnosis and Epidemiology of Rabies
Rodney E. Rohde and Bonny C. Mayes
 
15    Pyrosequencing for Bacterial Identification
Laura Millecker and Ruth Ann Luna
 
16    Other Pathogens of Significant Public Health Concern
Chris L. McGowin, Rodney E. Rohde, and Greg C. Whitlock
 
 
 
Section III Clinical Molecular Oncology Testing
 
17    Gene Rearrangements Testing
Siby Sebastian, Juan Du, and Rizwan Naeem
 
18    Mutation Analysis in Cancer
Keyur Pravinchandra Patel, John Galbincea,  and Rajyalakshmi Luthra
 
19    Minimal Residual Disease
Wesley Greaves and Rajyalakshmi Luthra
 
20    Pyrosequencing in Cancer
Bedia A. Barkoh, Rajyalakshmi Luthra, and Shalini Verma
 
21    Clinical Applications of Microarrays in Cancer
Marilyn M. Li, Ankita Patel, and Xiaofeng Hu
 
22    Basic Theories and Clinical Applications of Molecular Flow Cytometry
Hua Kang Wu, Hongli Chen, and Peter Hu
 
 
Section IV Clinical Molecular Pre/Postnatal Testing
 
23    Molecular Prenatal Diagnostic Testing
Joanna Wiszniewska and Christine M. Eng
 
24    PCR Based Diagnosis of Fragile X Syndrome 
Daniel J. Dykas and Allen E. Bale
 
 
Section V Human Identity Testing
 
25    Microsatellite Testing Short Tandem Repeat Profile Generation
Robin DeVille Guidry and Laura Gahn
 
26    Parentage
Laura Gahn and Robin DeVille Guidry
 
 
Section VI Clinical Molecular Training
 
27    Diagnostic Molecular Training
Peter Hu, Patrick A. Lennon, and Madhuri Hegde
 
28    Molecular Diagnostics Point of Care Testing Training
Chang W. Lee, and Peter Hu
 

Textul de pe ultima copertă

This timely book covers the need to know clinical practices for all those involved in molecular laboratory science. The field of molecular medicine is evolving at an astounding speed. Propelled by the new insights and technologies, advances are being made at an unprecedented rate. With dual measure given to today’s breakthroughs, this book is a collection of the most current practices relevant to the clinical molecular laboratorian. It begins with an introductory section on techniques and procedure.  It then presents four separate sections on infectious disease, oncology, pre/post-natal, and identity testing, with specific chapters clearly outlining clinical protocols used in daily practice.
Modern Clinical Molecular Techniques cuts to the heart of what is essential for the practicing molecular laboratory scientist. It is an outstanding resource for those operating within or looking to set up a clinical molecular laboratory.

Caracteristici

Presents the essentials of daily laboratory practice
Covers oncology testing, pre/post-natal testing, infectious disease testing, and identity testing
Details the most up-to-date protocols in molecular pathology
Written by the world’s foremost authorities in clinical molecular laboratory science