Molecular Diagnosis of Cancer: Methods and Protocols: Methods in Molecular Medicine, cartea 97
Editat de Joseph E. Roulston, John M. S. Bartletten Limba Engleză Paperback – 10 noi 2010
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Paperback (1) | 783.92 lei 43-57 zile | |
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Humana Press Inc. – 15 mar 2004 | 1104.13 lei 43-57 zile |
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Specificații
ISBN-13: 9781617373961
ISBN-10: 1617373966
Pagini: 408
Ilustrații: XII, 394 p.
Dimensiuni: 152 x 229 x 24 mm
Greutate: 0.54 kg
Ediția:Softcover reprint of hardcover 2nd ed. 2004
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Medicine
Locul publicării:Totowa, NJ, United States
ISBN-10: 1617373966
Pagini: 408
Ilustrații: XII, 394 p.
Dimensiuni: 152 x 229 x 24 mm
Greutate: 0.54 kg
Ediția:Softcover reprint of hardcover 2nd ed. 2004
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Medicine
Locul publicării:Totowa, NJ, United States
Public țintă
ResearchCuprins
Prognostic and Predictive Factors.- Assessment of Predictive Values of Tumor Markers.- Quality Assurance of Predictive Markers in Breast Cancer.- Extraction of Nucleic Acid Templates.- Microdissection and Extraction of DNA From Archival Tissue.- Fluorescence In Situ Hybridization.- HER2 FISH in Breast Cancer.- Fluorescence In Situ Hybridization for BCR-ABL.- UroVysion™ Multiprobe FISH in Urinary Cytology.- Chromogenic In Situ Hybridization in Tumor Pathology.- Comparative Genomic Hybridization and Fluorescence In Situ Hybridization in Chronic Lymphocytic Leukemia.- Molecular Characterization of Human Papillomaviruses by PCR and In Situ Hybridization.- A Nested RT-PCR Assay to Detect BCR/abl.- TP53 Mutation Detection by SSCP and Sequencing.- PCR Diagnosis of T-Cell Lymphoma in Paraffin-Embedded Bone Marrow Biopsies.- Circulating DNA Analysis.- Microsatellite Instability.- Diagnostic and Prognostic Significance of the Methylation Status of Myf-3 in Lymphoproliferative Disorders.- Quantitative Analysis of PRAME for Detection of Minimal Residual Disease in Leukemia.- Determination of Cyclin D1 Expression by Quantitative Real-Time, Reverse-Transcriptase Polymerase Chain Reaction.- Detection of Telomerase hTERT Gene Expression and Its Splice Variants by RT-PCR.- Detection of Telomerase Enzyme Activity by TRAP Assay.- Identification of TP53 Mutations in Human Cancers Using Oligonucleotide Microarrays.- Detection of K-ras Mutations by a Microelectronic DNA Chip.- Microarray-Based CGH in Cancer.- Tissue Microarrays.
Recenzii
"...concise and uniformly well written...contains a large compilation of applications of nucleic acid technologies that are in use or may be in use in the near future to diagnose and aid in the management of malignant diseases." - Doody's Health Sciences Book Review Journal
Review of the First Edition
The techniques described here are standard laboratory lore;the added value are the notes and tips on tricky details andoften overlooked precautions....Laboratory scientists...will get their money's worth. -Modern Pathology
"....a diverse collection of readily reproducible methods...the protocols presented follow step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls." - Tumori
From the reviews of the second edition:
"Dramatic advances in molecular biology over the past decades have led to an unprecedented understanding of cancer at a very detailed molecular level. … tips on troubleshooting are presented in each chapter, written by experts in the field. Given the amount of detail incorporated in each protocol, these should be readily reproducible and enable laboratories with no prior experience in a specific technique to master it and be able to use todays most powerful techniques in the molecular diagnosis of cancer." (Janis Racevskis, Medical Oncology, Vol. 22 (3), 2004)
Review of the First Edition
The techniques described here are standard laboratory lore;the added value are the notes and tips on tricky details andoften overlooked precautions....Laboratory scientists...will get their money's worth. -Modern Pathology
"....a diverse collection of readily reproducible methods...the protocols presented follow step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls." - Tumori
From the reviews of the second edition:
"Dramatic advances in molecular biology over the past decades have led to an unprecedented understanding of cancer at a very detailed molecular level. … tips on troubleshooting are presented in each chapter, written by experts in the field. Given the amount of detail incorporated in each protocol, these should be readily reproducible and enable laboratories with no prior experience in a specific technique to master it and be able to use todays most powerful techniques in the molecular diagnosis of cancer." (Janis Racevskis, Medical Oncology, Vol. 22 (3), 2004)
Textul de pe ultima copertă
The growing tide of novel therapeutics that target molecules within a specific individual's cancer cells-over 300 such agents are currently in phase I or II clinical trials-makes it essential to employ appropriate diagnostic methods and approaches when selecting patients for treatment. In Molecular Diagnosis of Cancer: Methods and Protocols, a panel of leading international experts in molecular diagnostic medicine present a diverse collection of readily reproducible methods, derived from their own research for cancer detection. Highlights include FISH-based methodologies currently used in the diagnosis of solid tumors, the molecular diagnosis of genetic abnormalities by DNA array technologies-including sequence-specific oligonucleotide arrays and CGH arrays-and methodologies directed at the detection of epigenetic events and at quantitative gene expression. The authors apply these novel diagnostic procedures to a broad range of cases taken from cytology, solid tumor pathology, hematology, and rare cell detection, paying special attention to potential future developments and the practical problems of dealing with quality control and accuracy. The protocols presented follow the successful Methods in Molecular Medicine™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Molecular Diagnosis of Cancer: Methods and Protocols offers pathologists providing molecular diagnostic services and researchers seeking molecularly targeted therapeutic approaches a broad array of today's most powerful methods and techniques.
Cutting-edge and highly practical, Molecular Diagnosis of Cancer: Methods and Protocols offers pathologists providing molecular diagnostic services and researchers seeking molecularly targeted therapeutic approaches a broad array of today's most powerful methods and techniques.
Caracteristici
Includes supplementary material: sn.pub/extras