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Nanoscopy and Multidimensional Optical Fluorescence Microscopy

Editat de Alberto Diaspro
en Limba Engleză Hardback – 26 apr 2010
"Alberto Diaspro has been choreographing light’s dance for over 20 years,
and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology’s secrets."
From the Foreword by Daniel Evanko, editor, Nature Methods


Nanoscopy and Multidimensional Optical Fluorescence Microscopy demonstrates that the boundaries between sciences do blur at the bottom, especially those that might separate the optical work of physicists and the cellular work of microbiologists. In 18 chapters written by pioneering researchers, this work offers the first comprehensive and current documentation of the cutting-edge research being accomplished in a wide range of photonic devices with revolutionary application.


The highlight of the book is its coverage of optical nanoscopy and super-resolution microscopy. The rapid advances in this area over the past few years offer researchers in both photonics and molecular biologya wealth of accomplishment upon which they can build.


Offering a complete treatment of this emerging field, this volume:




  • Describes how scientists have exploited the properties of light and its fluorophore partners to overcome the resolution limit of conventional light microscopy


  • Delves into recent ways to minimize the photobleaching that has long hampered many methods including those that have the potential to capture previously unobtainable information on the movements of single molecules


  • Discusses the principles, benefits, and implementation of fluorescence correlation spectroscopy and related methods, which simplifies analysis by limiting light to stationary focal points in a sample


  • Considers the most basic as well as emerging methods for improving three-dimensional optical sectioning microscopy


  • Reviews the basics of FRET (
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Specificații

ISBN-13: 9781420078862
ISBN-10: 1420078860
Pagini: 448
Ilustrații: 180 b/w images, 26 color images, 5 tables and Over 150
Dimensiuni: 178 x 254 x 25 mm
Greutate: 0.95 kg
Ediția:New.
Editura: CRC Press
Colecția Chapman and Hall/CRC

Public țintă

Academic and Professional Practice & Development

Cuprins

Optical Microsopy and Nanoscopy. Basis if Fluorescence Spectroscopy. Basis of Optics for Microscopy. Scanning Microscopy at the Nanoscale. 3D Computational Optical Sectioning Microscopy. Multiphoton Microscopy at the Nanoscale. Confocal Microscopy at the Nanoscale. 7D Microscopy. Lifetime Imaging Microscopy. Fluorescence Correlation Spectroscopy. FRET/FRAP. Single Molecule Microscopy. Single Particle Tracking (SPT). Fluorescent Probe. Fluorescence Photoactivation and Photoswitching. Fluorescent Nanotubes and Nanowires. Scattering Processes. SHG/THG (Second/Third Harmonic Generation) Microscopy. Near Field Microscopy. TIRF (Total Internal Reflection Fluorescence) Microscopy.

Descriere

A complete overview of the latest developments in optical microscopy that enable structure determination at the nanoscale, this timely book draws contributions from top experts in their respective fields, including those who have either led the current upsurge in research or who have laid the foundation for the development of modern microscopes and techniques. Contributors offer state-of-the-art treatment of critical topics in nanoscopy, from imaging and manipulation to spectroscopy and visualization. Interdisciplinary in scope and approach, the book combines the science and tools of biologists, physicists, and engineers.