Neuroanatomical Tract-Tracing: Molecules, Neurons, and Systems

Short Retrospection.- Preembedding Immunoelectron Microscopy: Applications for Studies of the Nervous System.- Postembedding Immunogold Cytochemistry of Membrane Molecules and Amino Acid Transmitters in the Central Nervous System.- Cell and Tissue Microdissection in Combination with Genomic and Proteomic Applications.- Molecules and Membrane Activity: Single-Cell RT-PCR and Patch-Clamp Recording from Central Neurons.- Merging Structure and Function: Combination of In Vivo Extracellular and Intracellular Electrophysiological Recordings with Neuroanatomical Techniques.- Juxtacellular Labeling of Individual Neurons In Vivo: From Electrophysiology to Synaptology.- Nonradioactive In Situ Hybridization in Combination with Tract-Tracing.- Viral Tracers for the Analysis of Neural Circuits.- Dextran Amines: Versatile Tools for Anterograde and Retrograde Studies of Nervous System Connectivity.- Multiple Neuroanatomical Tract-Tracing: Approaches for Multiple Tract-Tracing.- Tract-Tracing in Developing Systems and in Postmortem Human Material Using Carbocyanine Dyes.- Combined Fluorescence Methods to Determine Synapses in the Light Microscope: Multilabel Confocal Laser Scanning Microscopy.- Advances in Understanding Cortical Function Through Combined Voltage-Sensitive Dye Imaging, Whole-Cell Recordings, and Analysis of Cellular Morphology.- From Dendrites to Networks: Optically Probing the Living Brain Slice and Using Principal Component Analysis to Characterize Neuronal Morphology.- Stereology of Neural Connections: An Overview.- Three-Dimensional Computerized Reconstruction from Serial Sections: Cell Populations, Regions, and Whole Brain.- Atlases of the Human Brain: Tools for Functional Neuroimaging.- Neuron and Network Modeling.- Functional Connectivity of the Brain: Reconstruction from Static and Dynamic Data.

"The content of the book also serves to emphasize that neuroanatomy is, perhaps more than ever, a thriving and important part of the neurosciences...Most contributions have in common the combination of modern tract-tracing methods with other means of characterizing neural tissue, and thus the book also serves to highlight the gradual disappearance of borders between traditional neuroanatomy and other approaches to the study of the nervous system...this book should continue to be a useful source of information, and deserves to be available in all laboratories applying or considering to apply neuroanatomical methods."
P. Brodal, Neuroscience Vol.40, No. 1 

This revised edition details the new advances in molecular techniques, including genomics and proteomics, have developed into an important part of the neuroanatomical tract tracing, reviews the progress in fluorescence (including the development of new probes, microscopes, imaging equipment, and computers) Explains how the introduction of new markers has stimulated the field from tissue culture to neurophysiology to MRI techniques New edition is replete with detailed techniques and practical examples Includes supplementary material: sn.pub/extras