PCR Technology: Principles and Applications for DNA Amplification
Autor Henry Erlichen Limba Engleză Paperback – oct 1989
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Specificații
ISBN-13: 9780333489482
ISBN-10: 0333489489
Pagini: 246
Ilustrații: X, 246 p.
Dimensiuni: 152 x 229 x 15 mm
Greutate: 0.39 kg
Ediția:1989
Editura: Palgrave Macmillan UK
Colecția Palgrave Macmillan
Locul publicării:London, United Kingdom
ISBN-10: 0333489489
Pagini: 246
Ilustrații: X, 246 p.
Dimensiuni: 152 x 229 x 15 mm
Greutate: 0.39 kg
Ediția:1989
Editura: Palgrave Macmillan UK
Colecția Palgrave Macmillan
Locul publicării:London, United Kingdom
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Cuprins
Part 1 Introduction to the PCR: history of PCR development; description of the PCR method - basic principles and properties of the PCR; thermostable DNA polymerases; automation; sample preparation. Part 2 Research applications - brief overview: direct sequencing using PCR; preparation of specific DNA fragments using PCR; detection of gene expression; genetic mapping using PCR amplification of individual gametes. Part 3 Diagnostic applications - brief overview: detection of pathogens; diagnosis of monogenic genetic diseases; HLA DNA typing and disease susceptibility; forensic applications; detection of activated oncogenes; future developments and applications.