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Synthetic Biology, Part A: Methods for Part/Device Characterization and Chassis Engineering de Chris Voigt

Synthetic Biology, Part A: Methods for Part/Device Characterization and Chassis Engineering: Methods in Enzymology, cartea 497

Editat de Chris Voigt
en Limba Engleză Hardback – 8 iul 2011
Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation.


  • Encompasses a variety of different approaches, methodologies and disciplines
  • Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation
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Specificații

ISBN-13: 9780123850751
ISBN-10: 0123850754
Pagini: 714
Dimensiuni: 152 x 229 x 42 mm
Editura: ELSEVIER SCIENCE
Seria Methods in Enzymology

Public țintă

Researchers and students in cell, molecular and developmental biology

Cuprins

Section I. Measuring and Engineering Central Dogma Processes
  1. Sequence-specificity and Energy Landscapes of DNA-binding Molecules Joshua R. Tietjen, Leslie J. Donato, Devesh Bhimisaria and Aseem Z. Ansari
  2. Promoter Reliability in Modular Transcriptional Networks Rajat Anand, Navneet Rai and Mukund Thattai
  3. The Analysis of ChIP-seq Data Wenxiu Ma and Wing Hung Wong
  4. Using DNA Microarrays to Assay Part Function Virgil A. Rhodius and Carol A. Gross
  5. Orthogonal gene expression in Escherichia coli Wenlin An and Jason Chin
  6. Directed evolution of promoters and tandem gene arrays for customizing RNA synthesis rates and regulation Keith E.J. Tyo, Elke Nevoigt and Gregory Stephanopoulos
    Section II. Device and System Design, Optimization, and Debugging
  7. Design and Connection of Robust Genetic Circuits Ron Weiss
  8. Engineering RNAi circuits Yaakov Benenson
  9. From SELEX to cell: Dual selections for synthetic riboswitches Joy Sinha, Shana Topp, and Justin P. Gallivan
  10. Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells Jeffrey V. Wong, Guang Yao, Joseph R. Nevins and Lingchong You
  11. De novo design and construction of an inducible gene expression system in mammalian cells Maria Karlsson, Wilfried Weber and Martin Fussenegger
  12. BioBuilding: Using banana-scented bacteria to teach synthetic biology James Dixon and Natalie Kuldell
    Section III. Device Measurement, Optimization, and Debugging
  13. Use of Fluorescence Microscopy to Analyze Genetic Circuit Dynamics Gürol Süel
  14. Microfluidics for synthetic biology: From design to execution M. S. Ferry, I. A. Razinkov, J. Hasty
  15. Plate-based assays for light-regulated gene expression systems Jeffrey J. Tabor
  16. Spatiotemporal control of small GTPases with light using the LOV domain Yi I. Wu, Xiaobo Wang, Li He, Denise Montell and Klaus M. Hahn
  17. Light Control of Plasma Membrane Recruitment using the Phy—PIF system Jared E. Toettcher, Delquin Gong, Wendell A. Lim, Orion D. Weiner
  18. Synthetic Physiology: Strategies for Adapting Tools from Nature for Genetically-Targeted Control of Fast Biological Processes Ed Boyden
    Section IV. Devices for Metabolic Engineering
  19. Metabolic Pathway Flux Enhancement by Synthetic Protein Scaffolding John E. Dueber and Weston R. Whitaker
  20. A Synthetic Iterative Pathway for ketoacid elongation C. R. Shen, J. C. Liao
    Section V. Expanding Chasses
  21. Synthetic Biology in Streptomyces bacteria Marnix Medema, Rainer Breitling and Eriko Takano
  22. Methods for Engineering Sulfate Reducing Bacteria of the Genus Desulfovibrio Kimberly L. Keller, Judy D. Wall and Swapnil Chhabra
  23. Modification of the Genome of Rhodobacter sphaeroides and Construction of Synthetic Operons Paul R. Jaschke, Rafael G. Saer, Stephan Noll and J. Thomas Beatty
  24. Synthetic Biology in Cyanobacteria: Engineering and Analyzing Novel Functions Thorsten Heidorn, Daniel Camsund, Hsin-Ho Huang, Pia Lindberg, Paulo Oliveira, Karin Stensjö, and Peter Lindblad
  25. Developing a Synthetic Signal Transduction System in Plants Kevin J. Morey, Mauricio S. Antunes, Kirk D. Albrecht, Tessa A. Bowen, Jared F. Troupe, Keira L. Havens and June I. Medford
  26. Lentiviral Vectors to Study Stochastic Noise in Gene Expression
Kate Franz, Abhyudai Singh, and Leor S. Weinberger