The Molecular Biology of Insect Disease Vectors: A Methods Manual
Editat de J.M. Crampton, C.B. Beard, C. Louisen Limba Engleză Hardback – 31 dec 1996
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Specificații
ISBN-13: 9780412736605
ISBN-10: 0412736608
Pagini: 578
Ilustrații: XXV, 578 p. 41 illus., 1 illus. in color.
Dimensiuni: 216 x 280 x 38 mm
Greutate: 1.41 kg
Ediția:1997
Editura: SPRINGER NETHERLANDS
Colecția Springer
Locul publicării:Dordrecht, Netherlands
ISBN-10: 0412736608
Pagini: 578
Ilustrații: XXV, 578 p. 41 illus., 1 illus. in color.
Dimensiuni: 216 x 280 x 38 mm
Greutate: 1.41 kg
Ediția:1997
Editura: SPRINGER NETHERLANDS
Colecția Springer
Locul publicării:Dordrecht, Netherlands
Public țintă
ResearchCuprins
One Care and Maintenance of Insect Colonies.- 1 Care and maintenance of anopheline mosquito colonies.- 2 Care and maintenance of Aedes mosquito colonies.- 3 Care and maintenance of phlebotomine sandfly colonies.- 4 Care and maintenance of blackfly colonies.- 5 Care and maintenance of tsetse colonies.- 6 Care and maintenance of triatomine colonies.- Two Experimental Infection of Insect Vectors.- 7 Infection of mosquitoes with rodent malaria.- 8 Infection of mosquitoes with primate malaria.- 9 Infection of mosquitoes with filarial.- 10 Infection of phlebotomine sandflies with Leishmania.- 11 Infection of blackflies with Onchocerca.- 12 Infection of tsetse with Trypanosoma brucei rhodesiense and T. b. gambiense.- 13 Infection of triatomines with Trypanosoma cruzi.- Three Basic Methods in Isolating, Cloning and Characterizing Nucleic Acids and their Products.- 14 Isolation of genomic DNA.- 15 Isolation and analysis of RNA from insect vectors.- 16 Determination of insect genome size and complexity by reassociation kinetics.- 17 Constructing and screening lambda libraries.- 18 Constructing and screening cosmid libraries.- 19 Constructing and screening cDNA libraries.- 20 Southern/northern blotting and hybridization techniques.- 21 The polymerase chain reaction (PCR) and RT-PCR.- 22 Differential display of mRNA.- 23 Localization of RNA transcripts by in situ hybridization.- 24 In situ localization of proteins in whole mounted tissue.- 25 DNA sequencing and primer extension mapping.- Four Genome Mapping Techniques.- 26 RFLP analysis of insect genomes.- 27 Microsatellite mapping of insect genomes.- 28 Polytene chromosome preparation from anopheline mosquitoes.- 29 In situ hybridization to Anopheles polytene chromosomes.- 30 Microdissection and microamplification of dipteran polytene chromosomes.- Five Insect Identification Techniques.- 31 RAPD-PCR and SSCP analysis for insect population genetic studies.- 32 PCR amplification of insect ribosomal DNA.- 33 Generation and use of species-specific DNA probes for insect vector identification.- 34 Cloning and detection of insecticide resistance genes.- Six Transformation Techniques and Viral Systems.- 35 Microinjection of mosquito embryos.- 36 Transfection of cultured mosquito cells.- 37 Viral transducing vectors for mosquitoes.- 38 Viral expression systems and viral infections in insects.- Seven Cell and Organ Culture.- 39 Establishing primary cell cultures from disease vectors and maintenance of continuous cell lines.- 40 Culture and analysis of insect salivary glands.- 41 Culture and analysis of the insect fat body.- 42 Culture and analysis of insect ovaries.- 43 Culture and analysis of insect midgut.- Eight Insect Symbionts.- 44 Isolation and culture of tsetse secondary endosymbionts.- 45 Genetic manipulation of insect symbionts.- 46 PCR-based detection and identification of insect symbionts.