Volume Electron Microscopy: Methods in Cell Biology, cartea 177
Kedar Narayan, Lucy Collinson, Paul Verkadeen Limba Engleză Hardback – 22 iun 2023
Other sections focus on Mitochondria morphometry in 3d datasets of mouse brain obtained with serial block-face Scanning Electron Microscopy, Serial Block-face Scanning Electron Microscopy of Schmidtea mediterranea, Correlative multiscale microCT-SBF-SEM imaging of resin-embedded tissue, Methods of enhanced FIB-SEM sample preparation and image acquisition, Functional characterization of endo-lysosomal compartments by correlative live-cell and volume electron microscopy, and much more.
- Includes chapters written by key leaders and developers in the field
- Provides detailed protocols, allowing for the application of workflows in one’s own laboratory setting
- Presents real tips and tricks you won't get from standard research papers
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Specificații
ISBN-13: 9780323916073
ISBN-10: 0323916074
Pagini: 418
Dimensiuni: 191 x 235 mm
Greutate: 1.04 kg
Editura: ELSEVIER SCIENCE
Seria Methods in Cell Biology
ISBN-10: 0323916074
Pagini: 418
Dimensiuni: 191 x 235 mm
Greutate: 1.04 kg
Editura: ELSEVIER SCIENCE
Seria Methods in Cell Biology
Public țintă
This book is aimed at PhD students, post-doctoral researchers, and imaging facility staff working in the life science research area with an interest in microscopy technologies and applying or aiming to apply 3D ultrastructural techniques to their research.Cuprins
1. Automated large volume sample preparation for vEM
Claudia S. Lopez
2. Resin comparison for Serial Block Face Scanning Volume Electron Microscopy
Anneke Kremer, Saskia Lippens and Chris Geurin
3. Immunolabelling for SBF-SEM
Alice Liang
4. Volume Electron Microscopy in Plants
Kirk James Czymmek
5. Serial section electron tomography
Andrea Raimondi
6. Automated Tape Collecting Ultramicrotomy (ATUM) for targeting neuropathology
Martina Schifferer
7. Array tomography
Jemima J. Burden and Ian White
8. Mitochondria morphometry in 3d datasets of mouse brain obtained with serial block-face Scanning Electron Microscopy
Christel Genoud, Jean-Yves Chatton and Wei Jiao
9. Serial Block-face Scanning Electron Microscopy of Schmidtea mediterranea
Stephanie Nowotarski
10. Correlative multiscale microCT-SBF-SEM imaging of resin-embedded tissue
Rohan M. Lewis, Orestis L. Katsamenis and Pascale Chavatte-Palmer
11. Methods of enhanced FIB-SEM sample preparation and image acquisition
Song Pang
12. Functional characterization of endo-lysosomal compartments by correlative live-cell and volume electron microscopy
Nalan Liv
13. Cryo FIB-SEM imaging
Michele C. Darrow and James Naismith
14. Toward scalable reuse of vEM data: OME-Zarr to the rescue
Norman Rzepka, Joshua Allen Moore and John Bogovic
15. Public archiving of vEM data
Gerard J. Kleywegt and Ardan Patwardhan
Claudia S. Lopez
2. Resin comparison for Serial Block Face Scanning Volume Electron Microscopy
Anneke Kremer, Saskia Lippens and Chris Geurin
3. Immunolabelling for SBF-SEM
Alice Liang
4. Volume Electron Microscopy in Plants
Kirk James Czymmek
5. Serial section electron tomography
Andrea Raimondi
6. Automated Tape Collecting Ultramicrotomy (ATUM) for targeting neuropathology
Martina Schifferer
7. Array tomography
Jemima J. Burden and Ian White
8. Mitochondria morphometry in 3d datasets of mouse brain obtained with serial block-face Scanning Electron Microscopy
Christel Genoud, Jean-Yves Chatton and Wei Jiao
9. Serial Block-face Scanning Electron Microscopy of Schmidtea mediterranea
Stephanie Nowotarski
10. Correlative multiscale microCT-SBF-SEM imaging of resin-embedded tissue
Rohan M. Lewis, Orestis L. Katsamenis and Pascale Chavatte-Palmer
11. Methods of enhanced FIB-SEM sample preparation and image acquisition
Song Pang
12. Functional characterization of endo-lysosomal compartments by correlative live-cell and volume electron microscopy
Nalan Liv
13. Cryo FIB-SEM imaging
Michele C. Darrow and James Naismith
14. Toward scalable reuse of vEM data: OME-Zarr to the rescue
Norman Rzepka, Joshua Allen Moore and John Bogovic
15. Public archiving of vEM data
Gerard J. Kleywegt and Ardan Patwardhan