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A Practical Guide to Pharmacological Biotechnology de Jayanta Kumar Patra

A Practical Guide to Pharmacological Biotechnology: Learning Materials in Biosciences

Autor Jayanta Kumar Patra, Swagat Kumar Das, Gitishree Das, Hrudayanath Thatoi
en Limba Engleză Paperback – 4 apr 2019
Pharmacological biotechnology is applied to and used to study drug development, working mechanisms, diagnosis, and therapies. This textbook covers the whole range of experiments related to pharmacology. It also contains basic laboratory safety guidelines along with the basic calculations and formulas used in a laboratory. Each chapter starts with an introduction/theory into the basic approach followed by detailed methods sections with easy-to-follow protocols and comprehensive troubleshooting, calculations and possible questions for examination. The target group is researchers who are studying pharmacological biotechnology in the laboratory.
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Specificații

ISBN-13: 9789811363542
ISBN-10: 9811363544
Pagini: 132
Ilustrații: XIII, 142 p. 50 illus., 39 illus. in color.
Dimensiuni: 168 x 240 x 9 mm
Greutate: 0.27 kg
Ediția:1st ed. 2019
Editura: Springer Nature Singapore
Colecția Springer
Seria Learning Materials in Biosciences

Locul publicării:Singapore, Singapore

Cuprins

Part1.Introduction.- 1.1 Rules of laboratory safety in a pharmacology laboratory.- 1.2 Commom instruments and their uses used in a pharmacology laboratory.- Part2.General aspects of Pharmacology laboratory.- 2.1 Safety and Risk assessment.- 2.2 Use and Handling of laboratory animals.- 2.3 Experimental design.- 2.4 Essential statistics.- 2.5 Cumulative Dose Response Curve.- 2.6 Toxicology.- 2.7 Basic instruments and techniques in Pharmacology Laboratory .- Part3.Isolated tissues and organs.- 3.1 Basic instruments used for isolated tissue experiments.- 3.2 Organ baths.- 3.3 Smooth muscle preparations.- 3.4 Skeletal muscle preparations.- 3.5 Cardiac muscle preparations.- Part4.Isolated tissues for screening of drugs.- 4.1 Evaluation of antidiabetic agents.- 4.2 Evaluation of antidepressants.- 4.3 Evaluation of antihypertensive agents.- 4.4 Evaluation of antiulcer agents.- 4.5 Evaluation of hepatotoxicity.- 4.6 Evaluation of Antioxidant agents.- 4.7 Evaluation of local anaesthetics.-Part5.Genotoxicity and Toxicological studies.- 5.1 The Mouse Lymphoma Assay.- 5.2 The Comet Assay.- 5.3 In vitro Genotoxicity assay.- 5.4 In vitro Teratogenicity Testing.- 5.5 Histopathological studies of animal tissues.- 5.6 Drug poisoning.-  Part6.Experimental Animal studies.- 6.1 Pyrogen testing.- 6.2 Collecting blood from mice.- 6.3 Studies on different parameters of blood.- 6.4 Experiment on central nervous system.- 6.5 Evaluation on cardiovascular system.- 6.6 Experiments on GI tract.- Part7.Clinical trials.- 7.1 Clinical Pharmacology and its genesis.- 7.2 National and International agencies and their role in Clinical pharmacology.- 7.3 Stages in drug development and clinical trials.- 7.4 Ethics in Clinical research.- 7.5 Safety assessment in Clinical trials.- Part8.IPR and ethics in animal studies.- 8.1 Intellectual property rights and its different categories.- 8.2 Importance of IPR in drug development.- 8.3 Patenting cells, cell lines and animals.- 8.4 Ethics in laboratory animal studies .-  8.5 Risk assessment and management.- 8.6 Good laboratory practices.

Notă biografică

Jayanta Kumar Patra, Research Institute of Biotechnology & Medical Converged Science, Dongguk University, Republic of Korea
Swagat Kumar Das, Department of Biotechnology, College of Engineering and Technology, Biju Patnaik University of Technology, India
Gitishree Das, Research Institute of Biotechnology & Medical Converged Science, Dongguk University, Republic of Korea
Hrudayanath Thatoi, Department of Biotechnology, North Orissa University, India

Textul de pe ultima copertă

Pharmacological biotechnology is applied to and used to study drug development, working mechanisms, diagnosis, and therapies. This manual is the textbook covering the whole range of experiments related to pharmacology. It also contains basic laboratory safety guidelines along with the basic calculations and formulas used in a laboratory. Each chapter starts with an introduction/theory into the basic approach followed by detailed methods sections with easy-to-follow protocols and comprehensive troubleshooting, calculations and possible questions for examination. The target group is researchers who are studying pharmacological biotechnology in the laboratory.

Caracteristici

Offers practical guidelines for experiments in pharmacological biotechnology Addresses principles with workable protocols Illustrates flow-chart and troubleshooting for each experiment