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Animal Cell Culture: Principles and Practice de Shalini Mani

Animal Cell Culture: Principles and Practice: Techniques in Life Science and Biomedicine for the Non-Expert

Autor Shalini Mani, Manisha Singh, Anil Kumar
en Limba Engleză Hardback – 2 feb 2023
This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more. 
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Specificații

ISBN-13: 9783031194849
ISBN-10: 3031194845
Pagini: 327
Ilustrații: XVI, 327 p. 91 illus., 85 illus. in color.
Dimensiuni: 155 x 235 mm
Greutate: 0.66 kg
Ediția:1st ed. 2023
Editura: Springer International Publishing
Colecția Springer
Seria Techniques in Life Science and Biomedicine for the Non-Expert

Locul publicării:Cham, Switzerland

Cuprins

1. Overview to animal cell culture.- 2. Cell culture laboratory.- 3. Good lab practices and bio-safety measures.- 4. Managing sterility in the lab.- 5. Media and buffer preparation for cell culture.- 6. Properties of cultured cells and selection of culture media.- 7. Selection and maintenance of cultured cells.- 8. Inoculation and passaging of adherent cells.- 9. Counting of cells.- 10. Cryopreservation of cell lines.- 11. Resuscitation of frozen cell line.- 12. Isolation and culturing  of cells from different tissues.- 13. Differentiation, transformation and immortalization of cells.- 14. Co-culture techniques.- 15. 3d cell culture techniques.- 16. Stem cell culture techniques.- 17. Identification and removal of biological contamination in the media and cell suspensions.- 18. Analysis of cell growth kinetics in suspension and adherent types cell line.-  19. Applications of animal cell culture based assays.- 20. Ethical issues in animal cell culture.- 21. Common troubleshooting methods in cell culture techniques.

Notă biografică

Dr. Shalini Mani is Associate Professor in the Department of Biotechnology at Jaypee Institute of Information Technology, Noida, India. Dr. Manisha Singh is Assistant Professor and Lecturer in the Department of Biotechnology at Jaypee Institute of Information Technology, Noida, India. Dr. Anil Kumar is Director of Education and Professor at RLB Central Agricultural University, Jhansi, India. 

Caracteristici

Includes best practices and lab safety, ethical considerations, and troubleshooting for common issues Perfect for junior researchers, research students, and established scientists seeking a go-to quick guide One-stop reference or standard animal cell culture techniques